氨茶碱对慢性间断低氧大鼠气道炎症及肺组织氧化损伤拮抗作用的探讨

目的：探讨慢性低氧致气道炎症和肺组织氧化损伤的作用及氨茶碱对其的拮抗作用。方法： 成年雄性Wistar大鼠34只，随机分3组：对照组（n=10）、低氧组（n=12）、氨茶碱干预组（n=12）。检测各组大鼠血液及肺组织匀浆中肿瘤坏死因子-α（TNF-α） 、白细胞介素-10（IL-10）、脂质过氧化物（LPO）含量及超氧化物歧化酶（SOD）活性。结果： 与对照组相比，低氧组大鼠血液及肺组织匀浆TNF-α、IL-10、LPO含量显著增高（P<0.01），SOD活性显著降低（P<0.01），同时，肺组织匀浆TNF-α/IL-10增大（P<0.01）；与低氧组相比，氨茶碱干预组大鼠血液及肺组织匀浆TNF-α、LPO 含量显著降低（P<0.01），SOD活性、IL-10水平显著增高（P<0.01和P<0.05）。结论： （1）慢性低氧可引起气道炎症并介导肺组织氧化损伤；（2）氨茶碱具有抗炎和抗氧化作用。

Antagonistic effects of aminophylline on airway inflammation and oxidative lung tissue damage in chronic hypoxic rats

AIM: To investigate the effects of chronic hypoxia and antagonistic effects of aminophylline on airway inflammation and oxidative lung damage in rats. METHODS: Thirty-four male Wistar rats were randomly divided into three groups: normal control group (n=10); hypoxia group (n=12); aminophlline-treated group (n=12). The last two groups were both exposed to hypoxia 7 hours per day for 21 days. The third group was treated with aminophlline (100 mg·kg-1·d-1) before exposed to hypoxia. The level of tumor necrosis factor (TNF) -α, interleukin (IL)-10, lipid peroxide (LPO) and the activity of superoxide dismutase (SOD) were determined in blood and homogenates of lung tissue. RESULTS: Compared to the control group, the levels of TNF-α, IL-10 and LPO were significantly increased (P<0.01), the activity of SOD was significantly decreased (P<0.01) both in blood and homogenate of lung tissue. The ratio of TNF-α and IL-10 was significantly increased (P<0.01) in homogenate of lung tissue in hypoxia group. Compared to the hypoxia group, the levels of TNF-α, LPO and the ratio of TNF-α and IL-10 were significantly decreased (P<0.01) in blood and homogenate of lung tissue in aminophylline treated group, while the level of IL-10 and activity of SOD was significantly increased (P<0.05, P<0.01, respectively). CONCLUSIONS: Chronic hypoxia induces airway inflammation. Aminophylline produces anti-inflammatory effects on airway and anti-oxidantive effects on lung.