宫颈鳞状上皮癌蜡块中 HPV16 感染检测方法的比较研究

分别用原位杂交（ＩＳＨ）、多聚酶链反应（ＰＣＲ）和原位多聚酶链反应（ＩＳ－ＰＣＲ）方法，检测３９例宫颈鳞状上皮癌石蜡切片中ＨＰＶ的感染。ＩＳＨ的检出率为４６．２％（１７／３９），明显低于ＩＳ－ＰＣＲ的６６．７％（２６／３９，Ｐ＜０．０１）和ＰＣＲ的８４．６％（３３／３９，Ｐ＜０．０１）。用常规ＩＳＨ和地高辛标记探针能检出宫颈鳞状上皮癌ＨＰＶ１６感染的表皮细胞，而用ＩＳ－ＰＣＲ方法在同样标本中可显示出更多的ＨＰＶ１６阳性表皮细胞，并且着色更深。ＰＣＲ方法敏感性最高，但它不能用于组织细胞内的ＨＰＶ１６ＤＮＡ精确定位。

Comparation of Three Methods for theDetection of Human Papillomavirus DNAin Paraffin embedded Tissues of CervicalSquamous Intraepithelial Lesions

In order to compare the sensivity of three methods for the detection of human papillomavirus (HPV) 16 DNA, in situ hybridization (ISH), in situ hybridization after amplification by polymerase chain reaction (IS PCR) and polymerase chain reaction (PCR) were performed in paraffin embedded tissues of 39 cervical squamous intraepithelial lession (SILs). The results showed the detective rate of HPV DNA16 by ISH was 46.2% (17/39).It was lower than 66.7%(26/39) by IS PCR (P<0.05), and significantly lower than 84.6%(33/39) by PCR (P<0.01). Using conventional ISH with a dig labbeled probe,variable numbers of superfical cells in SILs showed detectable HPV DNA when in situ assay was performed after amplification,increased numders of superfical cells had detectable HPV16 DNA and the signals of hybridization were much more intense.PCR method showed the highest sensitivity,but it failed to show the precise localization of intracellular HPV16 DNA.