褪黑素联合顺铂对人食管癌Eca109细胞的抑制作用及其机制

目的:观察褪黑素(Mel)联合顺铂(DDP)对人食管癌Eca109细胞生长的抑制作用,并探讨相关的分子机制。方法:通过MTT实验检测Mel与DDP不同浓度分别单用或两药联合处理后食管癌Eca109细胞增殖情况;流式细胞仪检测用药后细胞凋亡情况;Western blot法检测Bax、Bcl-2蛋白的表达。结果:Mel与DDP联用对食管癌Eca109细胞增殖的抑制率均高于单用Mel与DDP时对食管癌Eca109细胞的抑制率(P<0.05);Mel单独诱导食管癌Eca109细胞凋亡作用不强,但可增强DDP诱导的食管癌细胞凋亡(P<0.01);Mel与DDP联用后食管癌Eca109细胞Bcl-2蛋白表达减弱,Bax蛋白表达增强。结论:Mel可增强DDP对人食管癌Eca109细胞增殖的抑制作用,并具有增强DDP诱导人食管癌Eca109细胞凋亡的作用,其机制可能与上调Bax表达和下调Bcl-2的表达有关。

Effect of Melatonin combined with cisplatin on proliferation and apoptosis of human esophageal carcinoma 109 cells

Objective:To explore the effects of melatonin combined with cisplatin on the proliferation and apoptosis of Eca109 cell and study the molecular mechanism. Methods:Eca109 cells were con-cultured with different concentrations of melatonin with or without cisplatin for 24 h. The inhibitory rate of Eca109 cells were examined using MTT assay,and the cell apoptosis was analyzed using flow cytometry with propidium iodide staining. Western blot for apoptosis-related protein Bcl-2 and Bax were used to analyze possible mechanisms of the synergistic anti-proliferation effect of melatonin in combination with cisplatin. Results:Different concentrations of melatonin combined with cisplatin showed an obviously enhanced inhibitory effect on colony formation of Eca109 cells. When melatonin combined with cisplatin,the cell apoptosis rate increased to 29%,significantly higher than that in cells with cisplatin treatment alone (9. 7%)(P<0. 01). The expression of Bcl-2 was high but Bax was low in Eca109 cells,after stimulating with melatonin and cisplatin, the expression of Bcl-2 decreased but expression of Bax increased. Conclusions:Melatonin can enhance the anti-proliferative effect of cisplatin on Eca109 cell and can enhance cisplatin-induced apoptosis. The possible mechanism may related to the downregulation of Bcl-2 and upregulation of Bax protein expression.