检测体内异种II型胶原异体移植引起的细胞毒反应

检测用猪II型胶原免疫新西兰大白兔的异种异体细胞免疫反应。用II型胶原免疫新西兰大白兔60 d,定期抽取血浆检测抗II型胶原抗体;第60 d取兔的外周血淋巴细胞、兔脾细胞、淋巴结分别分离淋巴细胞,进行体外二次II型胶原刺激,检测由此引起的反应性的细胞增殖规律。实验分为二组,第一组加入不同浓度植物血凝素(PHA)作阳性对照,并测定非特异性免疫;第二组加入不同浓度II型胶原,检测特异性免疫。正常兔的淋巴细胞在PHA剌激下发生增殖,但对II型胶原的第一次剌激不发生增殖,而免疫兔对PHA和II型胶原的剌激均能发生显著的增殖。表明异种II型胶原在一定浓度下,可以引起免疫兔的抗II型胶原抗体的升高,并可引起兔脾、外周血淋巴细胞增殖,异种II型胶原能在体内引起细胞免疫反应。

Detection of Heterogeneous Type II Collagen Transplantation Mediated Cytotoxic Response In Vitro

To detect the cellular immunity state of New Zealand white rabbit immunized by pig type(II) collagen.The New Zealand white rabbit was immunized by type II collagen for sixty days.The plasma was collected at a regular interval and the anti-type II collagen antibodies were examined.At the sixtieth day,the peripheral circular lymphocytes and the lymphocytes separated from spleen cells of rabbit and lymph nodes were collected and were stimulated by type II collagen in vivo again.The regulation of reactive cellular proliferation caused by the stimulation was detected.The experiment samples were divided into two groups.The first group was the positive control group by adding different concentrations of PHA and the non-specific immunity was assayed.The different concentrations of type II collagen were added to the second group and the specific immunity was assayed.The lymphocytes of normal rabbits showed proliferation by PHA stimulation but no proliferation by the first stimulation of type II collagen.Obvious proliferation due to the stimulation of both PHA and type(II) collagen in the immunized rabbit were observed.It shows that certain concentration of heterogeneous collagen may cause an increase of anti-type II collagen antibody in immunized rabbit and may cause a proliferation of lymphocytes in rabbit spleen and peripheral blood.The heterogeneous type II collagen causes cellular immunity in vivo.