卵磷脂胆固醇酰基转移酶基因511C/T多态性与脑出血的关联研究

目的 探讨卵磷脂胆固醇酰基转移酶(LCAT)基因511C/T多态性在中国汉族人群中的分布及其与脑出血的关联. 方法 应用聚合酶链反应(PCR)、单链构象多态性技术(SSCP)和DNA测序法检测中南大学湘雅医院神经内科自2004年10月至2005年12月收治的150例脑出血患者和122名年龄、性别相匹配的正常对照者的LCAT基因511C/T多态性. 结果 LCAT基因第四外显子511位点存在多态现象,此多态位点C/T在脑出血组和对照组均符合Hardy-Weinberg平衡定律.脑出血组基因型频率CT型为3.3%,T等位基因频率为1.7%;对照组基因型频率CT型为1.6%,T等位基因频率为0.8%;脑出血组CT基因型频率、T等位基因频率与对照组相比较差异无统计学意义(P＞0.05).脑出血组中511CC亚组高密度脂蛋白胆固醇(HDL-C)水平(1.44±0.42)mmol/L]明显高于511CT亚组(1.06±0.30)mmol/L],比较差异有统计学意义(P＜0.05). 结论 LCAT基因511C/T多态性可能与中国汉族人群脑出血发病无关,T等位基因可能与HDL-C代谢有关.

Abstract：

Objective To explore the distribution of lecithin cholesterol acyltransferase (LCAT)gene 511C/T polymorphism in Chinese Han population and the association of its polymorphism with the occurrence of cerebral hemorrhage (CH). Methods The LCAT gene 511C/T polymorphism was identified by PCR, single-strand conformation polymorphism (SSCP) and DNA sequencing in 150 patients with CH and 122 age- and gender-matched healthy controls. Results The 511 situs of the fourth extron in LCAT gene existed polymorphism; C/T in this situs of both groups satisfied Hardy-Weinberg equilibrium. The CT genotype frequency (3.3%) and T allele frequency (1.7%) in patient group were not significantly higher than those (1.6%, 0.8%, respectively) in controls (P＞0.05).The level of high-density lipoprotein cholesterol (HDL-C) in 511CC subgroup (1.44±0.42] mmol/L) was significantly higher than that in 511CT subgroup (1.06±0.30] mmol/L) of patient group (P＜0.05).Conclusion The LCAT gene 511C/T polymorphism is not possibly a predisposing factor of CH in Chinese Han population. T allele frequency might be connected with the metabolism of HDL-C.

Lecithin-cholesterol acyltransferase gene 511C/T polymorphism is associated with cerebral hemorrhage

Objective To explore the distribution of lecithin cholesterol acyltransferase (LCAT)gene 511C/T polymorphism in Chinese Han population and the association of its polymorphism with the occurrence of cerebral hemorrhage (CH). Methods The LCAT gene 511C/T polymorphism was identified by PCR, single-strand conformation polymorphism (SSCP) and DNA sequencing in 150 patients with CH and 122 age- and gender-matched healthy controls. Results The 511 situs of the fourth extron in LCAT gene existed polymorphism; C/T in this situs of both groups satisfied Hardy-Weinberg equilibrium. The CT genotype frequency (3.3%) and T allele frequency (1.7%) in patient group were not significantly higher than those (1.6%, 0.8%, respectively) in controls (P＞0.05).The level of high-density lipoprotein cholesterol (HDL-C) in 511CC subgroup (1.44±0.42] mmol/L) was significantly higher than that in 511CT subgroup (1.06±0.30] mmol/L) of patient group (P＜0.05).Conclusion The LCAT gene 511C/T polymorphism is not possibly a predisposing factor of CH in Chinese Han population. T allele frequency might be connected with the metabolism of HDL-C.