纳米金对表阿霉素的增敏作用

目的 观察纳米金对表阿霉素在抑制HepG2细胞增殖中如何发挥增敏作用.方法 实验组分为纳米金预处理组和单纯表阿霉素组.常规消化HepG2细胞后种板,各组分别加入2 μg/L的纳米金溶液和无血清培养液100μl,于设定的时间点(30、60、120和240 min)加入1 mg/L的表阿霉素溶液100μl,继续培养24 h后噻唑蓝(MTT)比色法检测两组药物对HepG2细胞的增殖抑制作用;紫外-可见分光光度计(UV-Vis)检测各组HepG2细胞内积聚的表阿霉素量;原子力显微镜(AFM)观察HepG2细胞表面形态及超微结构变化.结果　纳米金预处理组各时间点HepG2细胞增殖抑制率[(50.53±1.38)%、(51.83±0.47)%、(48.66±2.21)%、(43.55±1.01)%]均明显高于对应的单纯表阿霉素组[(37.24±3.49)%、(39.42±2.28)%、(34.98±2.27)%、(28.92±3.80)%],P值＜0.01;纳米金预处理组(60min)表阿霉索在HepG2细胞内积聚的量最多,为(4.01±0.14)μg;AFM检测到纳米金预处理组HepG2细胞膜内陷,粗糙度增加,表面孔径增大,细胞核的饱满程度减少.结论　纳米金可通过增加表阿霉素在HepG2细胞内的积聚量来发挥增敏作用,纳米金预处理60 min对表阿霉素的增敏作用最大.

Abstract：

Objectiye To observe the sensitization of gold nanoparticles on the epirubicin therapy of hepatocellular carcinoma cells (HepG2) and action mechanism. Methods The HepG2 cells were divided into 2 experimental groups: gold nanoparticles pretreatment group and simple epirubicin group. After seeded in a 96-well plate and cultured for 24 h, HepG2 cells were treated with 100 μl of gold nanoparticles (2 μg/L) and serum-free medium respectively. Then 100 μl of epirubicin ( 1 mg/L) was added to both groups at different time points: 30, 60, 120 and 240 min. The inhibition effect of epirubicin on the HepG2cells was assessed by methyl thiazol tetrazolium (MTT) assay. Ultraviolet-visible spectrophotometer (UV-Vis) was applied to detect the epirubicin accumulation in HepG2 cells at different time points, and atomic force microscope (AFM) was used to examine the ultrastructural changes of HepG2 cell surface. Results The inhibition rate of HepG2 cells in gold nanoparticles pretreatment group [(50. 53 ± 1.38 ) %,(51.83 ± 0. 47 ) %, (48. 66 ± 2.21 ) %, (43.55 ± 1.01 ) %] at different time points was higher than the simple epirubicin group remarkably [( 37.24 ± 3.49 ) %, ( 39. 42 ± 2. 28 ) %, ( 34. 98 ± 2. 27 ) %,(28.92 ±3. 80)%] ,P ＜0. 01. The gold nanoparticles pretreatment group (60 rain) had the maximal epirubicin accumulation: (4. 01 ±0. 14) μg. AFM revealed that when treated with gold nanoparticles, the morphologic changes of HepG2 cells were significantly different: there appeared invagination, obvious shrinked cell membrane and much rougher surface. Conclusion Gold nanoparticles can sensitize epirubicin through the enhancement of epirubicin accumulation in HepG2 cells. The pretreatment of gold nanoparticles for 60 min has the maximum sensitization.

The sensitization of gold nanoparticles on epirubicin

Objectiye To observe the sensitization of gold nanoparticles on the epirubicin therapy of hepatocellular carcinoma cells (HepG2) and action mechanism. Methods The HepG2 cells were divided into 2 experimental groups: gold nanoparticles pretreatment group and simple epirubicin group. After seeded in a 96-well plate and cultured for 24 h, HepG2 cells were treated with 100 μl of gold nanoparticles (2 μg/L) and serum-free medium respectively. Then 100 μl of epirubicin ( 1 mg/L) was added to both groups at different time points: 30, 60, 120 and 240 min. The inhibition effect of epirubicin on the HepG2cells was assessed by methyl thiazol tetrazolium (MTT) assay. Ultraviolet-visible spectrophotometer (UV-Vis) was applied to detect the epirubicin accumulation in HepG2 cells at different time points, and atomic force microscope (AFM) was used to examine the ultrastructural changes of HepG2 cell surface. Results The inhibition rate of HepG2 cells in gold nanoparticles pretreatment group [(50. 53 ± 1.38 ) %,(51.83 ± 0. 47 ) %, (48. 66 ± 2.21 ) %, (43.55 ± 1.01 ) %] at different time points was higher than the simple epirubicin group remarkably [( 37.24 ± 3.49 ) %, ( 39. 42 ± 2. 28 ) %, ( 34. 98 ± 2. 27 ) %,(28.92 ±3. 80)%] ,P ＜0. 01. The gold nanoparticles pretreatment group (60 rain) had the maximal epirubicin accumulation: (4. 01 ±0. 14) μg. AFM revealed that when treated with gold nanoparticles, the morphologic changes of HepG2 cells were significantly different: there appeared invagination, obvious shrinked cell membrane and much rougher surface. Conclusion Gold nanoparticles can sensitize epirubicin through the enhancement of epirubicin accumulation in HepG2 cells. The pretreatment of gold nanoparticles for 60 min has the maximum sensitization.