完全去交感肾上腺素能神经支配对小鼠前列腺癌生物学行为的影响

目的 观察完全去交感肾上腺素能神经支配对C57BL/6小鼠前列腺癌生物学行为的影响.方法 雄性10周龄C57BL/6小鼠50只,平均分为2组.小鼠前列腺双侧背侧叶包膜下注入RM-1细胞0.5×106个,然后切断(实验组)或不切断(对照组)其双侧下腹下神经,术后6 d始,动态观察小鼠前列腺癌局部生长、盆腔淋巴结转移、远处转移和荷瘤生存期;切断的神经纤维行酪氨酸羟化酶免疫组织化学染色.结果 切断的神经纤维组织切片酪氨酸羟化酶免疫组织化学染色为强阳性.术后小鼠前列腺肿瘤形成并进行性增大,实验组与对照组小鼠前列腺体积术后6 d[(0.034±0.008)/(0.034±0.008)cm3]、9 d[(0.339±0.040)/(0.316±0.050)cm3]、12 d[(0.829±0.090)/(0.824±0.071)cm3]、15 d[(1.169±0.093)/(1.236±0.103)cm3],差异均无统计学意义(P＞0.05);术后12 d:实验组4例、对照组3例肿瘤侵犯盆壁肌肉,实验组3例、对照组4例出现盆腔淋巴结转移;术后15 d:两组均有4例肿瘤侵犯精囊、膀胱或发生盆腔淋巴结转移,对照组出现肝实质和肾盂转移各1例,实验组无远处转移病例发现.实验组和对照组小鼠荷瘤生存期分别为(15.80±0.84)d和(16.00±0.71)d,差异无统计学意义(P＞0.05).结论 完全去交感肾上腺素能神经支配对C57BL/6小鼠前列腺癌局部生长、侵袭和盆腔淋巴结转移等过程似无明显影响,对其远处转移可能有抑制作用.

Abstract：

Objective To investigate the effects of complete denervation of sympathetic adrenergic nerves on the biological behaviors of prostate cancer in C57BL/6 mice. Methods Fifty male C57BL/6 mice, 10 weeks old, were randomly divided into two groups. By using a micro-syringe, RM-1 cells (0. 5 ×106) were injected into bilateral dorsal prostate capsules of mice, at the same time, bilateral hypogastric sympathetic nerves of the mice were cut (experimental group) or not (control group). At the sixth day postoperation, 5 mice were sacrificed in each group every three days to observe the local growth, invasion and metastasis of prostate cancer to pelvic nodes and other organs. An immunohistochemical determination of the hypogastric nerves was made by using an antibody against tyrosine hydroxylase ( TH) , a marker for sympathetic nerves. At the 15th day, 10 mice left were fed to death to calculate the life span of tumor-bearing mice. Results The volume of the prostate was gradually increased and confirmed as prostate cancer histologically. The prostate volume in experimental group was (0. 034 ± 0. 008) , (0. 339 ±0. 040) , (0. 829 ±0. 090) , ( 1. 169 ±0. 093) cm3 on the day 6, 9, 12, 15 after operation, respectively,while that in control group was (0.034 ± 0. 008), (0. 316 ± 0. 050), (0. 824 ± 0.071), (1. 236 ±0. 103) cm3, respectively (all P ＞ 0. 05). At the 12th day, muscle tissues around the prostate invasion by prostate cancer were observed in 3 cases of experimental group and 4 cases of control group; at the 15th day, invasion of seminal vesicles and bladder was found in 4 cases of experimental group and 4 cases of control group. Three and 4 mice had metastasis of pelvic nodes at 12th day and 15th day respectively in the experimental group, and 4 and 4 respectively in control group. At the 15th day post operation, in control group, liver parenchyma metastasis and renal pelvis metastasis were observed in 1 and 1 case, respectively, while in experimental group, no distant metastasis was observed. The life span of tumor-bearing mice in the experimental and control groups was (15.80±0.84) and (16.00±0.71) days, respectively (P＞0. 05 ) . Conclusion Complete denervation of sympathetic adrenergic nerves seems to have no significant effects on the local growth, invasion and pelvic nodes metastasis of prostate cancer in C57BL/6 mice, but may have some inhibition effects on its distant metastasis.

Effects of complete denervation of sympathetic adrenergenic nerve on biological behaviors of prostate cancer in mice

Objective To investigate the effects of complete denervation of sympathetic adrenergic nerves on the biological behaviors of prostate cancer in C57BL/6 mice. Methods Fifty male C57BL/6 mice, 10 weeks old, were randomly divided into two groups. By using a micro-syringe, RM-1 cells (0. 5 ×106) were injected into bilateral dorsal prostate capsules of mice, at the same time, bilateral hypogastric sympathetic nerves of the mice were cut (experimental group) or not (control group). At the sixth day postoperation, 5 mice were sacrificed in each group every three days to observe the local growth, invasion and metastasis of prostate cancer to pelvic nodes and other organs. An immunohistochemical determination of the hypogastric nerves was made by using an antibody against tyrosine hydroxylase ( TH) , a marker for sympathetic nerves. At the 15th day, 10 mice left were fed to death to calculate the life span of tumor-bearing mice. Results The volume of the prostate was gradually increased and confirmed as prostate cancer histologically. The prostate volume in experimental group was (0. 034 ± 0. 008) , (0. 339 ±0. 040) , (0. 829 ±0. 090) , ( 1. 169 ±0. 093) cm3 on the day 6, 9, 12, 15 after operation, respectively,while that in control group was (0.034 ± 0. 008), (0. 316 ± 0. 050), (0. 824 ± 0.071), (1. 236 ±0. 103) cm3, respectively (all P ＞ 0. 05). At the 12th day, muscle tissues around the prostate invasion by prostate cancer were observed in 3 cases of experimental group and 4 cases of control group; at the 15th day, invasion of seminal vesicles and bladder was found in 4 cases of experimental group and 4 cases of control group. Three and 4 mice had metastasis of pelvic nodes at 12th day and 15th day respectively in the experimental group, and 4 and 4 respectively in control group. At the 15th day post operation, in control group, liver parenchyma metastasis and renal pelvis metastasis were observed in 1 and 1 case, respectively, while in experimental group, no distant metastasis was observed. The life span of tumor-bearing mice in the experimental and control groups was (15.80±0.84) and (16.00±0.71) days, respectively (P＞0. 05 ) . Conclusion Complete denervation of sympathetic adrenergic nerves seems to have no significant effects on the local growth, invasion and pelvic nodes metastasis of prostate cancer in C57BL/6 mice, but may have some inhibition effects on its distant metastasis.