肾综合征出血热循环内皮细胞及热休克蛋白70的检测

目的检测肾综合征出血热（HFRS）患者循环内皮细胞（CEC）的数量变化及热休克蛋白70（HSP70）表达的动态变化,探讨其在HFRS中的临床意义。方法 HFRS患者34例,其中轻症组20例,重症组14例,另以20例健康者作对照。每例均按病期采血,采用Percoll密度梯度离心法分离内皮细胞后,应用流式细胞技术检测CEC数量及HSP70的表达,并同期检测肾功能。结果在不同病期的HFRS患者中,CEC计数及HSP70的表达在发热期均已升高,在低血压期和少尿期持续高水平,至恢复期逐渐降低,其变化曲线与血尿素氨（BUN）的变化趋势相似。结论 HFRS患者急性期CEC数量和CEC中HSP70表达均增高,其增高程度与病期和临床类型有关,且与肾损害有相关性;动态检测HFRS患者CEC及HSP70水平,可作为HFRS病情的重要观察指标,同时也可为HFRS发病机制及防治研究提供线索。

Detection and clinical significance of circulation endothelial cells and heat-shock protein 70 in patients with hemorrhagic fever with renal syndrome

Objective To investigate the dynamic changes of circulation endothelial cells （ CEC ） number and the expression of heat-shock protein70 （ HSP70） in patients with hemorrhagic fever with renal syndrome （HFRS） and its clinical significance. Methods Thirty-four HFRS patients were assigned to moderate group （20 cases） and severe group （14 cases） according to their illness condition, and 20 healthy volunteers served as the controls. After the CEC were separated by using Percoll density gradient centrifugation method, the CEC counts and the HSPT0 level were determined with flow cytometry to observe the changes of CEC counts and HSPT0 in patients with HFRS, and blood urea nitrogen （BUN） were tested by auto-matic biochemical analyzer simultaneously. Results In patients with different stages of HFRS, the counts of CEC and the expression of HSP70 of patients with HFRS began to increase in the fever stage and maintain a high level in the hypotension and oliguria stage, and then decreased gradually in recovery phase, the changes curve of which was similar to that of BUN. Conclusions The CEC counts and the expression of HSP70 in CEC both increase in the acute stage in patients with HFRS and the increase extent are related with the stage and clinical type of HFRS and also related with renal injury;furthermore, continuous measurement of these indexes could be an important method to disease observation and could provide a clue to the research of pathogenesis, prevention and cure of HFRS.