金属硫蛋白对皮瓣存活及细胞凋亡基因的影响

目的 观察金属硫蛋白(MT)对细胞凋亡基因及皮瓣存活影响.方法 大鼠背部形成随意皮瓣,实验组予以MT,对照组予以生理盐水,术后10 d测皮瓣存活率,术后3 d行苏木素-伊红(HE)染色光镜检查,激光多普勒测血运,术后3 d测皮瓣细胞凋亡基因bcl-2、bax蛋白.结果 MT组皮瓣的存活率(69.88±3.12)%高于对照组(60.65±2.98)%(P＜0.01).MT组中性粒细胞较多,激光多普勒血流量相对值(LDF值)下降少(P＜0.01).bcl-2蛋白表达MT组(2.98±0.23)较对照组(1.24±0.11)高(P＜0.01).bax蛋白(0.09±0.02)较对照组(0.23±0.09)低(P＜0.01).结论 皮瓣术后MT促进bcl-2,抑制bax,从而提高皮瓣存活率.

Abstract：

Objective To investigate whether metallothionein (MT) influences necrosis and expression of bcl-2 and bax in random pattern skin flaps of rats. Methods After flap operation, the rats in MT group were injected with MT, and those in control group received an injection of drug-free saline. Survival rate of the flaps was measured and the changes in the tissue were observed under the light microscopy.By using Doppler, the blood flow of flap was measured. The expression of bcl-2 and bax was detected. Results The survival area in MT group (69. 88 ±3. 12)% was significantly greater (P＜0.01) than that in control group (60. 65 ± 2. 98) %. There were more inflammatory cells in control group than in MT group.LDF in MT group was significiently higher than that in control group (P ＜0.01 ) 1st h, 2nd h, 3rd h, 6th h,1 st day, 3rd day, 6th and 7th day postoperation and at the 10th day postoperation (P ＜ 0. 05 ). At the 3rd day postoperation, the expression of bcl-2 in MT group (2.98 ± 0. 23 ) was significantly higher than that in control group ( 1.24 ± 0. 11 ) ( P ＜ 0. 01 ). The expression of bax in MT group ( 0. 09 ± 0. 02 ) was significantly lower than that in control group (0. 23 ± 0. 09) ( P ＜ 0. 01 ). Conclusion MT can affect the expression of bcl-2 and bax and significantly promote survival of skin flap.

Effects of metallothionein on flap necrosis and apoptosis

Objective To investigate whether metallothionein (MT) influences necrosis and expression of bcl-2 and bax in random pattern skin flaps of rats. Methods After flap operation, the rats in MT group were injected with MT, and those in control group received an injection of drug-free saline. Survival rate of the flaps was measured and the changes in the tissue were observed under the light microscopy.By using Doppler, the blood flow of flap was measured. The expression of bcl-2 and bax was detected. Results The survival area in MT group (69. 88 ±3. 12)% was significantly greater (P＜0.01) than that in control group (60. 65 ± 2. 98) %. There were more inflammatory cells in control group than in MT group.LDF in MT group was significiently higher than that in control group (P ＜0.01 ) 1st h, 2nd h, 3rd h, 6th h,1 st day, 3rd day, 6th and 7th day postoperation and at the 10th day postoperation (P ＜ 0. 05 ). At the 3rd day postoperation, the expression of bcl-2 in MT group (2.98 ± 0. 23 ) was significantly higher than that in control group ( 1.24 ± 0. 11 ) ( P ＜ 0. 01 ). The expression of bax in MT group ( 0. 09 ± 0. 02 ) was significantly lower than that in control group (0. 23 ± 0. 09) ( P ＜ 0. 01 ). Conclusion MT can affect the expression of bcl-2 and bax and significantly promote survival of skin flap.