二硫代氨基甲酸吡咯烷提高甲氨蝶呤对人骨肉瘤MG-63细胞增殖活性和凋亡的影响

目的 观察二硫代氨基甲酸吡咯烷(PDTC)和甲氨蝶呤(MTX)对人骨肉瘤MG-63细胞凋亡和Livin表达的影响.方法 体外培养骨肉瘤MG-63细胞株,用0、50、100、200和400μmol/L的PDTC和MTX作用于骨肉瘤MG-63细胞24、48和72 h后,用噻唑蓝(MTT)比色法检测骨肉瘤MG-63细胞的增殖活性,用流式细胞仪测细胞的凋亡率,用Western blot检测各组细胞的Livin蛋白表达水平.结果 各组骨肉瘤MG-63细胞的增殖活性随着时间增加降低,同时细胞的凋亡率随着时间增加(P＜0.05),各组细胞中Livin蛋白的表达明显降低(P＜0.05).结论 PDTC能提高MTX对骨肉瘤MG-63细胞的凋亡,其机制可能与下调Livin表达,阻断了Livin介导的抗凋亡途径有关.

Abstract：

Objective To detect the effect of pyirolidine dithiocarbamate (PDTC) and methotrexate (MTX) on apoptosis and expression of livin of human osteosarcoma MG-63 cells. Methods MG-63 cells were cultured in vitro. At 24, 48 and 72 h after treatment of PDTC and MTX at different concentrations (0, 50, 100, 200 and 400 μmol/L) , MTT assay was used to observe the growth inhibition of MG-63 cells. The apoptosis was assessed by flow cytometry. The protein expression of livin was detected by Westem blotting. Results When PDTC and MTX were added, growth inhibition and increased apoptosis of MG-63 cells were detected. The protein expression level of livin was down-regulated obviously ( P ＜0. 05). Conclusion PDTC can promote the apoptosis of MTX-treated MG-63 cells, which may be correlated with down-regulation of the livin expression.

Effect of PDTC and MTX on apoptosis and expression of Livin of human osteosarcoma MG-63 cells

Objective To detect the effect of pyirolidine dithiocarbamate (PDTC) and methotrexate (MTX) on apoptosis and expression of livin of human osteosarcoma MG-63 cells. Methods MG-63 cells were cultured in vitro. At 24, 48 and 72 h after treatment of PDTC and MTX at different concentrations (0, 50, 100, 200 and 400 μmol/L) , MTT assay was used to observe the growth inhibition of MG-63 cells. The apoptosis was assessed by flow cytometry. The protein expression of livin was detected by Westem blotting. Results When PDTC and MTX were added, growth inhibition and increased apoptosis of MG-63 cells were detected. The protein expression level of livin was down-regulated obviously ( P ＜0. 05). Conclusion PDTC can promote the apoptosis of MTX-treated MG-63 cells, which may be correlated with down-regulation of the livin expression.