重度子痫前期患者血清作用下自然杀伤细胞对血管内皮细胞功能的影响

目的 研究在重度子痫前期患者血清作用下,自然杀伤(natural killer,NK)细胞对血管内皮细胞功能的影响.方法 2006年1月1日至2008年12月31日中国医科大学附属盛京医院产科重度子痫前期患者、正常妊娠妇女各15例,采用1∶1配比的病例一对照研究.20%重度子痫前期及正常妊娠者(对照组)血清孵育NK细胞20 h,加入人脐静脉血管内皮细胞培养体系中培养24 h.采用流式细胞仪进行凋亡检测,透射电镜下观察细胞形态,放射免疫方法检测NK细胞与人脐静脉血管内皮细胞培养上清液中内皮素-1水平.结果 重度子痫前期组早期凋亡细胞(Annexin V-FITC+/PI-)为(23.81±4.79)%,晚期凋亡细胞(Annexin V-FITC+/PI+)为(3.29±1.04)%,对照组分别为(16.59±5.13)%和(2.24±0.72)%,差异均有统计学意义(P＜0.01);组间坏死细胞(Annexin V-FITC-/PI+)差异无统计学意义.透射电镜重度子痫前期组可见核膜溶解、核碎裂等细胞凋亡表现,上清液中内皮素-1水平为(39.08±9.04) μg/ml,较正常妊娠组[(29.98±7.04) μg/ml]明显升高,差异有统计学意义(t=3.439,P=0.004).结论 重度子痫前期患者血清可活化NK细胞,导致血管内皮细胞凋亡增多,分泌缩血管活性物质——内皮素-1增多,进而导致高血压等临床症状.

Abstract：

Objective To investigate the effect of natural killer (NK) cells treated by serum of severe preeclampsia patient on the function of endothelial cells.Methods Fifteen patients with severe preeclampsia and 15 normal pregnant women from the Obstetrics department,Shengjing Hospital,China Medical University were admitted into this case-control study from January 1,2006 to December 31,2008.NK cells from healthy non-pregnant woman were incubated with 20% serum from severe preeclampsia patients or normal pregnant women for 20 hours.Then,human umbilical vein endothelial cells ( HUVEC) and serum-treated NK cells were co-incubated for 24 hours.Apoptosis of HUVEC was checked by flow cytometry and electronic microscope.Endothelin-1 (ET-1) levels in the supernatants of HUVEC and NK cells were examined by radioimmunologic method.Results In severe preeclampsia group,the percentage of early apoptosis cell (Annexin V-FITC+ /PI+ ) was (23.81±4.79)%,that of late apoptosis cell (AnnexinV-FITC+/PI+ ) was (3.29±1.04) %,while those were (16.59±5.13)% and (2.24±0.72)% respectively in normal pregnant group (P＜0.01).There was no significant difference in dead cells (Annexin V-FITC- /PI+ ).Under electronic microscope,typical morphologic changes of apoptosis were shown in severe preeclampsia group.Level of ET-1 in

Effect of natural killer cells treated by serum of severe preeclampsia patient on apoptosis and endothelin-1 secretion of endothelial cells

Objective To investigate the effect of natural killer (NK) cells treated by serum of severe preeclampsia patient on the function of endothelial cells.Methods Fifteen patients with severe preeclampsia and 15 normal pregnant women from the Obstetrics department,Shengjing Hospital,China Medical University were admitted into this case-control study from January 1,2006 to December 31,2008.NK cells from healthy non-pregnant woman were incubated with 20% serum from severe preeclampsia patients or normal pregnant women for 20 hours.Then,human umbilical vein endothelial cells ( HUVEC) and serum-treated NK cells were co-incubated for 24 hours.Apoptosis of HUVEC was checked by flow cytometry and electronic microscope.Endothelin-1 (ET-1) levels in the supernatants of HUVEC and NK cells were examined by radioimmunologic method.Results In severe preeclampsia group,the percentage of early apoptosis cell (Annexin V-FITC+ /PI+ ) was (23.81±4.79)%,that of late apoptosis cell (AnnexinV-FITC+/PI+ ) was (3.29±1.04) %,while those were (16.59±5.13)% and (2.24±0.72)% respectively in normal pregnant group (P＜0.01).There was no significant difference in dead cells (Annexin V-FITC- /PI+ ).Under electronic microscope,typical morphologic changes of apoptosis were shown in severe preeclampsia group.Level of ET-1 in