Subsets of retinal neurons and glia express P2Y1 receptors |
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Authors: | M.M. Ward E.L. Fletcher |
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Affiliation: | Department of Anatomy and Cell Biology, The University of Melbourne, Grattan Street, Parkville 3010, Victoria, Australia |
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Abstract: | Recent evidence suggests that extracellular ATP modulates retinal processing and could play a role in modulating glial cells during retinal diseases. Here, we evaluated the localization of P2Y1 receptors in the rat retina using indirect immunofluorescence immunocytochemistry. We observed labeling within defined populations of inner retinal neurons and Müller cell processes and end feet. Double labeling of P2Y1 receptor with choline acetyltransferase revealed extensive colocalization indicating the expression of this receptor by cholinergic amacrine cells. Ganglion cell labeling for P2Y1 receptors was also observed. Having established the normal pattern of immunolabeling within the retina, we next examined whether immunolabeling was altered by retinal disease. P2Y1 receptor immunolabeling of Müller cells was of greater intensity following light-induced retinal degeneration, suggesting that Müller cell gliosis is accompanied by changes in P2Y1 receptor expression. Overall, these data provide further evidence for a role of extracellular ATP in retinal signaling within subsets of retinal neurons as well as glia. |
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Keywords: | purinergic cholinergic amacrine cells Mü ller cells glial cell signaling neuromodulation astrocytes |
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