Neural correlates of scent marking behavior in C57BL/6J mice: detection and recognition of a social stimulus |
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Authors: | KG Borelli DC Blanchard LK Javier EB Defensor ML Brandão RJ Blanchard |
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Institution: | 1. Pacific Bioscience Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA;2. Department of Psychology, University of Hawaii at Manoa, 2430 Campus Road, Honolulu, HI 96822, USA;3. Department of Genetics, Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96822, USA;4. Laboratório de Psicobiologia, Faculdade de Filosofia e Ciências Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, 14040-901 Ribeirão Preto, SP, Brazil;5. Instituto de Neurociências and Comportamento-INeC, Campus USP, 14040-901 Ribeirão Preto, SP, Brazil |
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Abstract: | Mice show urinary scent marking behavior as a form of social communication. Marking to a conspecific stimulus mouse or odor varies with stimulus familiarity, indicating discrimination of novel and familiar animals. This study investigated Fos immunoreactivity in inbred C57BL/6J (C57) males following scent marking behavior in response to detection of a social stimulus, or discrimination between a familiar and an unfamiliar conspecific. In Experiment 1 C57 mice were exposed for four daily trials to an empty chamber; on a test day they were exposed to the same chamber or to a male CD-1 mouse in that chamber. Increased scent marking to the CD-1 mouse was associated with increased Fos-immunoreactive cells in the basolateral amygdala, medial amygdala, and dorsal and ventral premammillary nuclei. In Experiment 2 C57 mice were habituated to a CD-1 male for 4 consecutive days and, on the 5th day, exposed to the same CD-1 male, or to a novel CD-1 male. Mice exposed to a novel CD-1 displayed a significant increase in scent marking compared to their last exposure to the familiar stimulus, indicating discrimination of the novelty of this social stimulus. Marking to the novel stimulus was associated with enhanced activation of several telencephalic, as well as hypothalamic and midbrain, structures in which activation had not been seen in the detection paradigm (Experiment 1). These included medial prefrontal and piriform cortices, and lateral septum; the paraventricular nuclei, ventromedial nuclei, and lateral area of the hypothalamus, and the ventrolateral column of the periaqueductal gray. These data suggest that a circumscribed group of structures largely concerned with olfaction is involved in detection of a conspecific olfactory stimulus, whereas discrimination of a novel vs. a familiar conspecific stimulus engages a wider range of forebrain structures encompassing higher-order processes and potentially providing an interface between cognitions and emotions. |
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Keywords: | olfactory detection of conspecifics discrimination of social novelty social memory medial prefrontal cortex lateral septum amygdala |
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