一氧化碳对缺氧预适应小鼠缺氧诱导因子-1表达的影响

目的:探讨缺氧预适应小鼠中一氧化碳对缺氧诱导因子-1表达和缺氧耐受时间的影响。方法:小鼠腹腔注射生理盐水(NS)或氯化高铁血红素(hemin)后,进行重复缺氧4次实验,Western印迹法检测海马中缺氧诱导因子-1α(HIF-1α)的表达,并记录缺氧耐受时间。结果:缺氧1次和2次,注射NS和注射hemin小鼠海马中HIF-1α的水平基本相同,缺氧耐受时间相似。重复缺氧3次和4次,注射hemin小鼠海马中HIF-1α含量少于注射NS小鼠,缺氧耐受时间低于注射NS小鼠(P＜0.05)。结论:在缺氧预适应模型中,一氧化碳能减少海马中HIF-1α的表达,进而降低缺氧耐受时间。其机制可能为一氧化碳抑制血红素蛋白氧感受器的功能。

Effects of carbon monoxide on hypoxia inducible factor-1 expression in hypoxia preconditioned mice

AIM:To study the effect of carbon monoxide (CO) on hypoxia inducible factor-1α(HIF-1α) expression and on hypoxic tolerance duration in hypoxia preconditioned mice. METHODS:Mice were injected with normal saline (NS) or hemin intraperitoneally. Western blot was used to detect HIF-1α in hippocampus. The tolerant duration of each hypoxic run was recorded. RESULTS:After 1 and 2 runs of hypoxia exposure, there were no significant differences in HIF-1α expression and hypoxic tolerance duration between N and hemin injection groups. After 3 and 4 runs of hypoxia exposure, the content of HIF-1α was lower and hypoxic tolerance duration was shorter (P＜0.05) in hemin injection groups than that in NS injection groups. CONCLUSION:CO could decrease the expression of HIF-1α, and decrease hypoxic tolerance duration in hypoxia preconditioned mice. The mechanism may be that CO binds to hemoprotein and inhibits hemoprotein oxygen sensor.