孕鼠感染弓形虫对子代的影响

目的观察孕期感染弓形虫对子代鼠发育、寿命和免疫功能的影响。方法以不同剂量RH株弓形虫腹腔感染孕中期鼠(d10),分娩后观察记录子鼠发育过程中体长、体重、寿命;给予实验目的要求的干预措施,观察智力发育情况及免疫功能变化。结果弓形虫轻感组、重感组、对照组孕鼠分娩子鼠体长差异无显著性,体重、寿命、智力发育有差别;重感组子鼠寿命明显短于对照组和轻感组。轻感组子鼠智力干预前后差异有显著性,子鼠智力可以通过干预措施达到正常或得到改善,其他组无明显变化;受到再攻击感染时,重感染组子鼠感染率显著高于轻感组和对照组,且与IgG含量成反比。孕期弓形虫重度感染使子鼠抗感染能力下降,但血清特异性抗体IgG能起到一定的保护作用。结论孕期感染弓形虫出生子鼠体重、智力、免疫功能降低,寿命缩短,与感染剂量大小有关。对体长无明显影响。

THE EFFECT OF PREGNANT MICE INFECTED WITH TOXOPLASMA GONDII ON THEIR GENERATION

Objective To observe the effect of pregnant mice infected with Toxoplasma gondii on their generation's growth, life-span and immune functions. Methods The mid-period pregnant mice were infected with different doses of T. gondii RH strain by abdominal cavity. Post partum, the generation's body length, weight and life-span in development were observed. The intellectual development and immunity functions were detected after intervening measures were carried out according to experimental objectives. Results There was no difference in the body length among slightly infected group, seriously infected group and control group, but there was difference in their weight, life-span and intellectual development. The life-span of seriously infected group was shorter than that of lightly infected group and control group. There was significantly difference in intelligence in lightly infected group before and after intervention, the intelligence of young mice could reach normal or be improved by intervening measures. There was no change in other groups. The infection rate of seriously infected group was markedly higher than that of lightly infected group and control group after reinfection of T. gondii, and it was inversely proportional to IgG level. The young mice whose mother infected T. gondii seriously would lower its immunity function, but anti-body IgG could protect them from being infected again. Conclusion After pregnant mice infect T. gondii, its generation's weight, intelligence and immune function are lower, life-span is curtate, but there is no obviously effect on the body length of the three groups.