| 氨胺－T法和Iodogen法碘标记抗体的比较 |
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| 引用本文： | 刘长征,张汝森,张勇. 氨胺－T法和Iodogen法碘标记抗体的比较[J]. 中山大学学报(医学科学版), 1995, 0(3) |
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| 作者姓名： | 刘长征 张汝森 张勇 |
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| 作者单位： | 中山医科大学实验核医学教研室 |
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| 摘 要： | 本文比较了氯胺－Ｔ法和Ｉｏｄｏｇｅｎ法碘化标记单克隆抗体ＢＡＣ５在不同反应条件下的标记率，标记反应的时间与抗体免疫活性之间的关系。结果表明：这两种标记方法的最佳ｐＨ值为７～８；反应体积在１００～２００μｌ范围内均可以得到较好的结果；氯胺－Ｔ法反应３ｍｉｎ，标记率已接近最大值，而Ｉｏｄｏｇｅｎ法要在１０ｍｉｎ后才有最大的产额。结果还表明：在本实验条件下，氨胺－Ｔ法和Ｉｏｄｏｇｅｎ法的反应时间分别小于３ｍｉｎ和５ｍｉｎ时，其抗体的免疫活性可保留原来的９５％以上。
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| 关 键 词： | 同位素标记／方法，碘放射性同位素，抗体，单克隆，免疫活性 |
COMPATATIVE STUDY OF IODOGEN AND CHLORAMINE-TMETHODS FOR THE RADIOIODINATION OF ANTIBODY |
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| Liu Changzheng,Zhang Rusen,Zhang Yong. COMPATATIVE STUDY OF IODOGEN AND CHLORAMINE-TMETHODS FOR THE RADIOIODINATION OF ANTIBODY[J]. Journal of Sun Yatsen University(Medical Sciences), 1995, 0(3) |
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| Authors: | Liu Changzheng Zhang Rusen Zhang Yong |
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| Abstract: | he radioiodination technique has been applied extensively in the isotope labeling of antibodies.This study compared the radiolabeling yields of monoclonal antibody BAC5iodinated by iodongen and chloramine-T methods under different conditions and observed the relationship between the immunoativity and the labeling time.It has beenshown that the maximal yield occurred in the range of pH7-8 both methods,and the optimal reaction time(t)and volume(V)for chloramine-T and Iodogen methods were 3minutes(t), 500ul(V) and 10 minutes(t),between 100 and 300 l(V) respectively.95% of its original immunoactivity has been remained with the labeling time of less than 3 and 5 minutes by chloramine-T and Iodogen methods respectively. |
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| Keywords: | isotopes labeling/methods iodine radioisotopes antibodies monoclonal immunnocompetence |
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