细胞黏附分子(ICAM-1)在大鼠弥漫性脑损伤中的表达意义

目的探讨ICAM—1在大鼠弥漫性脑损伤中的表达及意义。方法采用Marmarou方法获得大鼠弥漫性脑损伤模型．实时定量RT—PCR、S—P免疫组化法分别测定ICAM—1蛋白和mRNA在外伤后不同时间点的表达变化．干湿重法测脑组织含水量．组织切片苏木精一伊红染色观测炎性细胞浸润情况。结果外伤组与假手术对照组比较，ICAM—1蛋白表达分别于伤后6h明显升高．72h达到高峰（P〈0.05），ICAM—ImRNA表达3h即明显升高，72h达最高峰，其后下降。7d时仍高于假手术对照组（P〈0．01）。伤后脑组织含水量较假手术对照组高，同时炎性细胞大量聚集。结论大鼠脑外伤诱导了ICAM—1的表达．ICAM—1通过介导炎性细胞的浸润可能参与了伤后脑水肿的形成过程．

Expression and significance of intercellular adhesion molecule-1 after diffuse brain injury in rats

Objective To explore the expression and significance of ICAM-1 after diffuse brain injury in rats. Methods The rat models of diffuse brain injury were established by Marmarou's method. Real-time quantitative PCR, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of ICAM-1, the change of mRNA at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury. Results The expression of ICAM-1 markedly increased 6h after injury in the injury groups in contrast to sham-operated group, peaked at the 72nd hour (P<0.05). ICAM-1 mRNA expression in the injury group markedly increased at the 3rd hour, peaked at the 72nd hour and was higher than that in sham-operated group at the 7th days (P<0.01). The water containing in the brain tissue after brain injury in the injury group was higher than that in the sham-operated group with prominent inflammatory cell infiltration in the brain tissue. Conclusion Traumatic brain injury could induce expression of ICAM-1, suggesting that ICAM-1 may contribute to the formation of brain edema by inducing infiltration of inflammatory cells into traumatic brain tissue.