香烟烟雾促进膀胱癌干细胞的自我更新和干性基因的表达

目的: 探讨香烟烟雾对膀胱癌干细胞自我更新能力和干性基因表达的影响。方法: 用不同浓度（0%、0.1%和0.25%）的香烟烟雾悬液处理人膀胱癌细胞UM-UC-3来源的膀胱癌干细胞4 d，显微镜下观察膀胱癌干细胞球大小及数量，蛋白质印迹检测干性基因CD44、ALDH1-A1、OCT-4、Nanog等的蛋白表达。结果： 0.1%和0.25%的香烟烟雾悬液能够促进膀胱癌干细胞细胞球的形成，香烟烟雾处理组的细胞球明显大于对照组；蛋白质印迹结果显示，0.1%和0.25%的香烟烟雾悬液处理的膀胱癌干细胞CD44、ALDH1-A1、OCT-4、Nanog表达显著增加。结论： 香烟烟雾能够促进膀胱癌干细胞的自我更新和干性基因的表达。

Tobacco smoke promoted the self-renewal ability and expression of stemness genes ofbladder cancer stem cells

Objective: To investigate the effect of tobacco smoke on self-renewal ability and stemness genes expression of bladder cancer stem cells. Methods: The bladder cancer stem cells obtained from human bladder cancer cells UM-UC-3 were treated with different concentrations of cigarette smoke extract(CSE, 0%，0.1% and 0.25%) for 4 days. The effect of CSE on the sizes and quantities of bladder cancer stem cells spheres were observed. The expression of stemness genes CD44, ALDH1-A1， OCT-4, and Nanog were detected by Western blotting.  Results: 0.1% and 0.25% CSE promoted the formation of bladder cancer stem cell spheres, while the cell spheres of CSE groups were significantly larger than the control group; Western blotting results showed that the expression of CD44, ALDH1-A1, OCT-4, and Nanog were significantly increased in 0.1% and 0.25% CSE groups. Conclusion: Tobacco smoke can promote the self renewal ability of bladder cancer stem cells and upregulate the expression levels of CD44, ALDH1-A1， OCT-4, and Nanog. ［Key words］cigarette smoke extract; bladder cancer stem cells; self-renewal; stemness genes