荷瘤小鼠MDSCs中TRIM25和PTEN的表达

目的: 探讨荷瘤小鼠肿瘤组织来源的髓源抑制性细胞（myeloid derived suppressor cells, MDSCs）中三基序蛋白25（tripartite motif containing 25, TRIM25）和人第10号染色体缺失的磷酸酶及张力蛋白同源基因（phosphatase and tensin homologue deleted on chromosome ten, PTEN）的表达水平及其意义。方法: 构建小鼠CT26结肠癌移植瘤模型，采用实时荧光定量PCR（real time fluorescene quantitative PCR, qRT PCR）技术检测荷瘤小鼠肿瘤组织来源MDSCs和野生型小鼠脾脏来源MDSCs中TRIM25、PTEN的表达情况。结果： 在小鼠CT26结肠癌移植瘤模型中，与野生型小鼠脾脏来源的MDSCs相比，荷瘤小鼠肿瘤组织来源MDSCs中TRIM25表达水平明显升高（P<0.001），PTEN表达水平显著下降（P<0.001）。结论： 荷瘤小鼠肿瘤组织来源MDSCs中TRIM25的表达升高，PTEN表达下降，提示两者或许参与调控MDSCs。

Expression of TRIM25 and PTEN in MDSCs of tumor-bearing mice

Objective: The study was to investigate the expression and significance of TRIM25 and PTEN in myeloid derived suppressor cells (MDSCs) from tumor tissue of tumor-bearing (TB) mice. Methods: CT26 colon cancer tumor-bearing mice models were established. The expressions of TRIM25 and PTEN were detected in MDSCs derived from tumor tissue of TB mice and spleen of wild-type (WT) mice with qRT PCR method. Results: In CT26 cells bearing mice models, the expression level of TRIM25 in tumor tissue-derived MDSCs of TB mice was significantly higher than that from spleen of WT mice(P<0.001). Inversely, compared to WT mice, PTEN expression was obviously down-regulated in MDSCs isolated from tumor tissue of TB mice (P<0.001). Conclusion: TRIM25 expression level was up-regulated, while the expression of PTEN was suppressed in MDSCs from tumor tissue of TB mice. They both may participate in the regulation of MDSCs.