高氧液对大鼠肝移植的保护作用

背景:目前可供移植的器官日益短缺,但因灌注冷保存所至的原发性移植物无功能仍然有一定的发生率,减少灌注保存所至的器官损伤有较大的临床意义.目的:观察高氧液对大鼠肝移植的保护作用.方法:选用Wistar大鼠40只,按随机数字表法分成2组,即高氧乳酸林格液组(高氧液组)和乳酸林格液(普通灌注液组),每组20只,各组供、受体各半,制备肝、肾、胰器官簇移植模型.高氧液组使用高氧液灌注,普通灌注液组使用普通林格液灌注,比较两组灌注末、肝移植后第1,3天透明质酸质量浓度、谷丙转氨酶活性、CD8+CD28-T细胞含量,以及移植后肝组织病理急性排斥评分.结果与结论:移植前两组透明质酸质量浓度、谷丙转氨酶活性、CD8+CD28-T细胞含量差异无显著性意义(P>0.05).移植后高氧液组透明质酸质量浓度、谷丙转氨酶活性均低于普通灌注液组(P<0.05),CD8+CD28-T细胞含量高于普通灌注液组(P<0.05),高氧液组Banff评分低于普通灌注液组(P<0.05).提示高氧液可减轻缺血再灌注损伤,对大鼠肝移植有一定的保护作用.

Protective effect of hyperoxic fluid on rat liver transplantation

BACKGROUND: Organ for transplantation is insufficient, and primary transplant of nonfunction caused by perfusion cryopreservation occasionally occurs. It is clinically significant to reduce organ damage caused by perfusion preservation. OBJECTIVE: To explore the protective effect of hyperoxic perfusion fluid on liver transplantation in rats. METHODS: A total of 40 Wistar rats were randomly divided two groups (n = 20) and respectively poured with Ringer lactate solution or hyperoxic ringer lactate solution. Each group comprised equal number of donors and recipients to prepare liver, kidney, and pancreas transplantation models. Hyaluronic acid (HA), alanine aminotransferase (ALT) and CD8+CD28- T cells were compared between two groups at the end of perfusion, and 1st and 3rd days after liver transplantation. The acute rejection score of liver tissues were also compared after operation. RESULTS AND CONCLUSION: The HA, ALT and CD8+CD28-T cells were no significantly different between two groups before operation (P> 0.05). The HA and ALT of hyperoxic ringer lactate solution group was significantly Ringer lactate solution group after liver transplant (P < 0.05), but the CD8+CD28-T cells were greater (P < 0.05). The acute rejection scores for liver in hyperoxia liquid group were significantly less than the common liquid group (P< 0.05). Results show that hyperoxic solution can attenuate ischemia/reperfusion injury and protect rats undergoing liver transplantation.