| 实验感染的猕猴血清和粪便中HEVRNA动态变化研究 |
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| 引用本文： | 李凡,庄辉,朱万孚,朱永红,郭晓霞. 实验感染的猕猴血清和粪便中HEVRNA动态变化研究[J]. 北京大学学报(医学版), 1995, 0(4) |
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| 作者姓名： | 李凡 庄辉 朱万孚 朱永红 郭晓霞 |
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| 作者单位： | 北京医科大学流行病学教研室 |
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| 摘 要： | 应用逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）法检测实验感染ＨＥＶ猕猴急性期血清及粪便标本中ＨＥＶＲＮＡ，并与其血清ＡＬＴ异常和抗－ＨＥＶＩｇＧ阳转相比较，以了解猕猴感染ＨＥＶ后上述各项标志的动态变化规律。结果表明，ＨＥＶＲＮＡ血症多出现于ＡＬＴ升高之前，持续约１～２周，当血清抗－ＨＥＶｌｇＧ阳转时ＨＥＶＲＮＡ即转为阴性；粪便ＨＥＶＲＮＡ可与病毒血症同时出现，但持续时间较病毒血症为长，于感染后３～４周仍可检测到。
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| 关 键 词： | 戊型肝炎病毒，PCR／方法 |
DYNAMICS OF HEV RNA IN SERA AND FAECES OF EXPERIMENTALLY INFECTED RHESUS MONKEYS |
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| Affiliation: | Department of Epidemio1ogy Li Fan,et al |
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| Abstract: | sensitive and specific RT-PCR assay was used for the detection of HEV RNA in sera and faeces of experi-mentally infected rhesus monkeys.The patterns of viraemia and virus shedding in faeces were compared with the dynamics of ALT elevation and seroconversion of anti-HFV IgG.The results showed that HEV RNA in serum was detected before the elevation of ALT and disappeared at or soon after seroconversion of anti-HEV IgG,with a peri-od of 1-2 weeks.Excretion of HEV RNA in stools was coincident with viraemia and remained positive for 2-4 weeks,indicating that HEV shedding in faeces is more prolonged than viraemia. |
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| Keywords: | Headings Hepatitis E virus PCR/methods |
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