超声击破微泡效应抑制兔VX_2肿瘤生长的实验研究

目的 探讨超声击破微泡效应抑制兔VX_2肿瘤生长的可行性.方法 21只移植有皮下VX_2肿瘤的新西兰大白兔,随机分为超声微泡治疗组、单纯超声组和假照组.经静脉注射脂质微泡的同时,脉冲式聚焦超声直接辐照肿瘤区域.单纯超声组和假照组分别用5 ml生理盐水和超声假照替代,每次10 min,每隔72 h治疗一次,采集各时间点肿瘤二维声像图和超声造影影像,测量肿瘤切面最大直径.结果 在30 d的实验周期内,微泡超声治疗组、单纯超声组和假照组的肿瘤平均直径分别从(1.1±0.1)cm、(1.2±0.1)cm、(1.2±0.1)cm生长至(2.1±0.5)cm、(3.0±0.9)cm、(3.4±0.7)cm,微泡超声治疗组肿瘤直径明显小于另两组.结论 超声击破微泡效应可阻断肿瘤微循环,有一定的抑制肿瘤生长作用.

Growth Inhibition of subcutaneous VX_2 tumor by ultrasound mediated microbubble disruption

Objective To investigate the feasibility of VX_2 tumor growth inhibition by using ultrasound mediated microbubble disruption.Methods Twenty-one New Zealand white rabbits bearing subcutaneous VX_2tumor were randomly divided into 3 groups for the impact factors including microbubbles(MB)and ultrasound(US).Pulsed focused ultrasound was delivered directly to the tumor surface for ten minutes following intravenous infusion of microbubbles in the experimental(US+MB)group.The two control groups were applied with only saline injection and sham US exposure.The procedure was repeated every 72 hours until the 30th day.Contrast and grey scale ultrasonography were acquired after every treatment to get the tumor perfusion and measurement.Results The diameters were sized every 72h in a course of 30 days.The average maxium diameter of the US+MB,US,and SHAM groups increased from(1.1±0.1)cm,(1.2±0.1)cm,(1.2±0.1)cm to(2.1±0.5)cm,(3.0±0.9)cm,(3.4±0.7)cm,respectively.The maximal diameter of the US+MB group was significantly smaller than those of the two control groups.Conclusions The VX_2 tumor growth can be inhibited by ultrasound mediated microbubbte disruption effects.