| 多聚乙酰亚胺介导CNP基因对兔髂动脉球囊成形术后内膜增生的影响 |
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| 引用本文： | 肖乐,龚昆梅,刘为军,王昆华,欧阳一鸣,凌平,LI Lin-hai.多聚乙酰亚胺介导CNP基因对兔髂动脉球囊成形术后内膜增生的影响[J].昆明医学院学报,2011,32(8):3-8. |
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| 作者姓名： | 肖乐 龚昆梅 刘为军 王昆华 欧阳一鸣 凌平 LI Lin-hai |
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| 作者单位： | 昆明医学院附属昆华医院,云南省第一人民医院普通外科,云南昆明650032 |
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| 基金项目： | 国家自然科学基金资助项目(30860276); 云南省应用基础研究计划资助项目(2007M272); 云南省科技厅-昆明医学院联合专项基金资助项目(2008CD042) |
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| 摘 要： | 目的观察局部转染C型利钠肽基因（CNP）对血管内膜增生的影响,探讨CNP在防治球囊成形术后再狭窄中的作用.方法构建兔髂动脉粥样硬化模型,将多聚乙酰亚胺-pcDNA3.1/hCNP包被球囊转染髂动脉,分别用RT-PCR、免疫组化、Western blot等方法检测pcDNA3.1/hCNP在动脉壁中的表达,弹力纤维染色法检测球囊成形术后内膜增生等指标.结果 pcDNA3.1/hCNP在兔髂动脉中能高效表达,转染组血管内膜增生程度明显降低.结论 CNP基因转染能明显抑制血管内膜增生,在血管再狭窄防治中可能具有应用价值.
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| 关 键 词： | C型利钠肽 基因治疗 血管再狭窄 |
The Influence of C-type Natriuretic Peptide Gene induced by Polyethylenimine on the Intima Hyperplasia at Injured Vessels after Iliac Aortic Sac Plasty in Rabbit Model |
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| XIAO Le,GONG Kun-mei,LIU Wei-jun,WANG Kun-hua,OUYANG Yi-ming,LING Ping,LI Lin-hai.The Influence of C-type Natriuretic Peptide Gene induced by Polyethylenimine on the Intima Hyperplasia at Injured Vessels after Iliac Aortic Sac Plasty in Rabbit Model[J].Journal of Kunming Medical College,2011,32(8):3-8. |
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| Authors: | XIAO Le GONG Kun-mei LIU Wei-jun WANG Kun-hua OUYANG Yi-ming LING Ping LI Lin-hai |
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| Institution: | XIAO Le,GONG Kun-mei,LIU Wei-jun,WANG Kun-hua,OUYANG Yi-ming,LING Ping,LI Lin-hai(Dept.of General Surgery,The First People's Hospital of Yunnan Province,The Affiliated Kunhua Hospital of Kunming Medical University,Kunming Yunnan 650032,China) |
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| Abstract: | Objective To discuss the potential role of C-type natriuretic peptide(CNP)in vascular restenosis prevention after aortic sac plasty by observating the influence of CNP on the intima hyperplasia.Methods Reconstructed plasmid pcDNA3.1/hCNP was transfected with polyethylenimine(PEI)by catheter method in iliac artery of rabbit model of atherosclerosis.The expression of pcDNA3.1/hCNP was confirmed with RT-PCR,immunohistochemistry and Western blot.The hyperplasia of intima was detected with elastic fibers stainin... |
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| Keywords: | C-type natriuretic peptide Gene therapy Restenosis |
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