单侧髁突切除对非手术侧髁突软骨细胞增殖与凋亡的影响

目的研究兔单侧髁突切除对非手术侧髁突软骨细胞增殖和凋亡活性的影响.方法 24只5～6月龄的日本大耳白兔被随机分为两组：空白对照组和手术组.手术组通过手术切除右侧髁突建立动物模型,分别于术后2月、4月的时间处死动物,取其左侧（非手术侧）颞颌关节,采用免疫组化染色和TUNEL染色,分别观察非手术侧髁突软骨中增殖细胞核抗原（PCNA）及凋亡细胞的表达和分布.结果免疫组化和TUNEL染色显示与同时间段空白对照组相比,无论是手术组（单侧髁突切除）术后2月,还是手术组术后4月,非手术侧髁突软骨PCNA阳性细胞表达量均降低;而TUNEL染色阳性细胞数增高（P〈0.05）;与手术组术后2月相比,手术组术后4月PCNA阳性细胞表达量降低;而TUNEL染色阳性细胞数增高（P〈0.05）.结论单侧髁突切除可导致非手术侧髁突软骨细胞增殖能力下降,凋亡细胞数量增加.

Effect of Unilateral Condylectomy on the Proliferation and Apoptosis of the Opposite Condylar Chondrocytes

Objective To investigate the effect of unilateral condylectomy on the proliferation and apoptosis of the opposite condylar chondrocytes.Methods Twenty-four 5～6 months old Japanese big-ear rabbits were randomly divided into 2 groups：the blank control group and the operation group.Rabbits in the opetation group were performed removing the right condylar.The left condylar（the nonoperative side）cartilages of each group were dissected in 2 months and 4 months after the operation accordingly.Immunohistochemical staining was used to qualitively examine the PCNA positive cell.TUNEL was chosen to detect the apoptotic cell.Results Immunohistochemical staining and TUNEL showed that： compared with the blank control group at the same time-points,the number of the PCNA positive cells decreased significantly in both 2 months and 4 months operation group;while the number of TUNEL positive cells increased significantly in both 2 months and 4 months operate group（P0.05）.Compared with the operation group in 2 months after the operation,the number of the PCNA positive cells decreased significantly in the operation group in 4 months after the operation;while the number of TUNEL positive cells increased significantly（P0.05）.Conclusion Unilateral condylectomy can decrese the proliferation capacity and increase apoptosis of the opposite condylar chondrocytes.