| 肺腺癌A549细胞株细胞活力变化及GDNF表达 |
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| 引用本文： | 谭燕,袁兵,王盛兰,赵珊,王廷华,张云辉.肺腺癌A549细胞株细胞活力变化及GDNF表达[J].昆明医学院学报,2011,32(8):38-42. |
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| 作者姓名： | 谭燕 袁兵 王盛兰 赵珊 王廷华 张云辉 |
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| 作者单位： | 1. 云南省第一人民医院呼吸内科,云南昆明,650000
2. 昆明医学院神经科学研究所,云南昆明,650031 |
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| 摘 要： | 目的探讨GDNF在肺腺癌A549细胞株的表达及细胞活力变化.方法体外培养肺腺癌A549细胞株,观察细胞形态变化.取第1代培养后1 d,5 d两个时间点分别计数细胞;MTT检测两时间点细胞活力变化;用免疫组化检测两时间点GDNF免疫阳性细胞数量变化.并进行统计学分析.结果培养5 d组肺腺癌A549细胞株的细胞数量与1 d组相比,明显增加（P〈0.05）;5 d组的细胞活力较1d组明显下降（P〈0.05）;GDNF的表达百分数比较显示;5 d组亦较1 d组明显下降（P〈0.05）.结论体外培养肺腺癌A549细胞株随细胞增殖过程,活力逐渐下降,同时GDNF表达明显下调,提示细胞增殖和细胞活力不一定完全一致,GDNF减少可能与活力减少有关.
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| 关 键 词： | GDNF A549 肺腺癌 |
Expression of GDNF and Changes of Cell Viability of Lung Adenocarcinoma Cell Lines A549 |
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| TAN Yan,YUAN Bing,WANG Sheng-lan,ZHAO Shan,WANG Ting-hua,ZHANG Yun-hui.Expression of GDNF and Changes of Cell Viability of Lung Adenocarcinoma Cell Lines A549[J].Journal of Kunming Medical College,2011,32(8):38-42. |
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| Authors: | TAN Yan YUAN Bing WANG Sheng-lan ZHAO Shan WANG Ting-hua ZHANG Yun-hui |
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| Institution: | 1)Dept. of Respiration,The First People’s Hospital of Yunnan Province,Kunming Yunnan 65000;2)Institute of Neuroscience,Kunming Medical University,Kunming Yunnan 6500031,China) |
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| Abstract: | Objective To study the expression and cell viability changes of GDNF in lung adenocarcinoma cell lines A549.Methods Lung adenocarcinoma cell lines A549 were cultured and morphological changes were observed.Cells were counted and cell viability were detected by MTT method after culturing for 1d and 5 d.timultaneously,GDNF positive cells were detected by immunohistochemistry and statistically analyzed.Results The cell number of lung adenocarcinoma cell lines A549 was significantly increased in 5 d group,compa... |
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| Keywords: | GDNF A549 Lung adehocacinoma |
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