| 电刺激大鼠小脑顶核对缺血-再灌注视网膜的保护作用实验研究 |
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| 引用本文： | 罗哲文,张虹. 电刺激大鼠小脑顶核对缺血-再灌注视网膜的保护作用实验研究[J]. 昆明医学院学报, 2011, 32(10): 41-44 |
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| 作者姓名： | 罗哲文 张虹 |
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| 作者单位： | 1. 云南省第二人民医院眼科,云南昆明,650021
2. 华中科技大学同济医学院附属同济医院眼科,湖北武汉,430030 |
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| 摘 要： | 目的探讨电刺激大鼠小脑顶核（cerebellar fastigial nucleus,CFN）对视网膜缺血-再灌注损伤的保护作用及其机制.方法大鼠随机均分为三组,缺血-再灌注损伤组、缺血-再灌注治疗组和假手术组.用TUNEL试剂盒检测视网膜神经节细胞凋亡率;用免疫组织化学染色法检测视网膜细胞Bcl-2蛋白和Bax蛋白的表达.结果缺血-再灌注损伤可使视网膜神经节细胞发生凋亡（P〈0.05）,同时视网膜细胞Bcl-2蛋白表达逐渐减弱（P〈0.05）,Bax蛋白表达逐渐增强（P〈0.05）;而使用电刺激小脑顶核的治疗组上述改变较轻（P〈0.05）.结论电刺激大鼠小脑顶核对视网膜缺血-再灌注损伤具有保护作用,其机制可能通过上调Bcl-2蛋白表达,下调Bax蛋白表达来减少视网膜细胞的凋亡.
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| 关 键 词： | 缺血-再灌注 电刺激 小脑顶核 凋亡 TUNEL Bcl-2 Bax 视网膜 |
Electrical Stimulating Cerebellar Fastigial Nucleus Protects Retina against Ischemia/reperfusion Injury in Rats |
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| LUO Zhe-wen,ZHANG Hong. Electrical Stimulating Cerebellar Fastigial Nucleus Protects Retina against Ischemia/reperfusion Injury in Rats[J]. Journal of Kunming Medical College, 2011, 32(10): 41-44 |
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| Authors: | LUO Zhe-wen ZHANG Hong |
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| Affiliation: | LUO Zhe-wen1),ZHANG Hong2)(1)Dept.of Ophthalmology,The Second People's Hospital of Yunnan Province,Kunmin Yunnan 650021,2)Dept.of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430030,China |
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| Abstract: | Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats.Methods Rats were divided randomly into three groups:group1(ischemia-reperfusion),group2(electrical-stimulating-treated),and group3(sham-operated).Apoptosis of retinal ganglion cells was detected by TUNEL method,and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods.Results Ischemia/reperfusion injury induced ... |
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| Keywords: | Ischemia-reperfusion injury Electrical stimulation Cerebellar fastigial nucleus Apoptosis TUNEL Bcl-2 Bax Retina |
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