体外受精—胚胎移植在不孕症治疗中的应用—附50个取卵周期结果分析

目的：明确不孕类型．促排卵方案和培养方法对体外受精的影响情况。方法：50个取卵周期，45个周期进行了胚胎移植，获得13例临床妊娠，移植周期妊娠率29％。44例不孕患者，21例为原发性不孕症，23例为继发性不孕症。卵巢刺激方案采用垂体降调节后卵巢刺激方案和不含GnRHa卵巢刺激方案。应用Earle’s培养基培养胚胎。结果：原发性不孕症每病例妊娠率14．29％，继发性不孕症每病例妊娠率为43．48％。比较垂体降调节后卵巢刺激方案和不含GnRHa卵巢刺激方案，促性腺激素的用量和用药时间统计学上无差异显著性。结论：原发性不孕症体外受精妊娠率低于继发性不孕症，选择合适的培养方法和超排方案可能会提高体外受精妊娠率。

CLINICAL APPLICATION OF IN VITRO FERTILIZATION FORINFERTILITY: ANALYSIS OF 50 OOCYTE RETRIEAL CYCLES

Aim: The impact of type of in fertility, protocol of ovarian stimulation and culture method on the procedure of in vitro fertilization. Methods: 21 out of 44 cases were patients with primary in fertility, 23 cases with secondary infertility.The protocol of ovarian stimulation was protocol without GnRHa or blocking protocoI with GnRHa. Embryos were cultured in Earle's medium. Results: 45 cycles out of total 50 cycles were completed. 13 cases got pregnancy and total clinical pregnancy rate per transfer was 29%. Clinical pregnancy rate per case for primary in fertility group was 14. 29%, 43. 48% for sec-ondary infertility group. Blocking protocol with GnRHa and protocol without GnRHa for twarian stimulation were compared.There were no statistical significence in duration and amount of gonadotropins. Conclusions: Pregnancy rate of IVF with pri-mary infertility group was much less than that with secondary in fertility group. The outcome of IVF may be improved through selecting proper culture method and stimulating protocol.