miRNA在小鼠着床前胚胎的表达

目的:研究小鼠发育过程中miRNA表达水平的变化,探讨miRNA对着床前胚胎发育过程的作用。方法:建立小鼠超排、交配系统,收集着床前胚胎提取低分子量RNA;采用miRNA特异RT引物进行反转录,采用SYBR Green实时定量PCR技术,研究miR-125a、miR-295和miR-181b在小鼠着床前胚胎发育过程中表达水平的变化。结果:着床前胚胎发育的各阶段均表达miR-125a、miR-295和miR-181b;随着胚胎发育进程,miR-295的表达呈逐渐上升的趋势;miR-125a在卵母细胞到2细胞发育阶段呈下降趋势,以后随发育进程呈逐渐上升趋势。结论:小鼠着床前胚胎中表达miRNA,着床前胚胎的发育和分化过程可能受到miRNA的调控。

Expression profilings of miRNA in mouse preimplantation embryos

Objective：To investigate the expression of miRNAs in mouse preimplantation embryos and to elucidate the functions of miRNA on mouse embryo development. Methods：Mouse preimplantation embryos were collected after superovulation, miRNA specific RT primers were used to reverse｜y transcribe the miRNAs including miR-125a,miR-295 and miR-181b, then miRNAs were amplified by SYBR Green real-time PCR using miRNA specific PCR primers. Results ：miR-125a,miR-295 and miR-181b were all expressed in mouse preimplantation embryos. With the embryo development, the expression of miR-295 was increased sharply and the expression of miR-125a was waved in differential stage of embryo development. Conclusion： The present study suggests that mouse preimplantation embryos express miRNAs involved with development and cell differentiation. These miRNAs might influence developmental and/or differential processes of mouse preimplantation embryos by regulating mRNA associated with embryo development.