黑色素瘤抗原基因MAGE-1、MAGE-3和抑癌基因p53在肺癌中表达的研究

目的:分析肺癌组织MAGE-1和MAGE-3基因以及p53基因表达及其相互关系。方法:取肺癌组织标本30例,另取癌周组织作为对照。采用逆转录聚合酶链反应(RT-PCR)方法,检测MAGE-1和MAGE-3基因以及p53基因的表达。结果:MAGE-1,MAGE-3在肺癌中有较高表达,MAGE-3的表达高于MAGE-1的表达。p53在肺癌中表达较低,在正常组织中表达较高;MAGE-1在肺腺癌中表达较鳞癌高,MAGE-3在肺鳞癌中表达较高;MAGE-1,MAGE-3的表达可能与肿瘤分化程度无关;p53在小细胞肺癌中的表达有待进一步研究;p53与MAGE在体内表达成负相关。结论:MAGE-1、MAGE-3和p53在肺癌组织与正常组织中有不同的表达,MAGE-1、MAGE-3的表达与病理类型有关,与肿瘤分化无关。

Expression of MAGE-1,MAGE-3 and p53 genes in lung carcinomas

Objective:In this study,we analyzed the expression of MAGE-1,MAGE-3 and p53 genes in lung cancers and assessed the relationship among them.Methods:Using polymerase chain reaction(RT-PCR) methods,we studied 60 specimen that were obtained from surgical samples or fiber bronchoscope(FB) biopsy including 30 lung carcinoma and 30 matched adjacent normal tissues.Results:MAGE-1 and MAGE-3 genes showed high expression in lung cancer.The expression of MAGE-3 gene was higher than that of MAGE-1 gene in lung cancer.p53 gene expression is low in lung carcinomas but high in normal tissues ;The expression of MAGE-1 gene was higher in adenocarcinomas than that in squamous cell carcinomas.Otherwise the expression of MAGE-3 gene was higher in squamous cell carcinomas than that in adenocarcinomas ;There is no direct relation between the tumor differentiation degree and the expression of MAGE-1,MAGE-3 genes.The expression of p53 possibly needs more studies in small cell lung cancer;The expression between p53 gene and MAGE genes probably had inverse correlation.Conclusion:The expression lecels of MAGE-1,MAGE-3 and p53 genes are different in lung carcinoma and normal tissues.The expression of MAGE-1,MAGE-3 are related to the pathological and not related to the tumor differentiation degree.