大鼠角膜中央区SP样、CGRP样免疫阳性纤维的起源

向角膜中央区注射ＨＲＰ后，用抗ＳＰ和抗ＣＧＲＰ抗体对三叉神经节、颈上神经节和迷走神经节进行免疫组织化学处理。结果证明：双重阳性细胞出现于三叉神经节的内侧区且集中分布于背内侧部．ＣＧＲＰ样双重阳性细胞主要分布在节的背内侧部的周边区；ＳＰ样双重阳性细胞散在于节的背内侧部的深部。两者均为圆形或椭圆形。在颈上神经节和迷走神经节内未见双重阳性细胞。

ORIGINS OF SP-LI FIBRES AND CGRP-LI FIBRES OF CENTRAL AREA OF CORNEA OF RAT

The corneal nerve regeneration and corneal sensation recovery following corneal surgery have been a difficult problem. So far, however, the origins of corneal afferent nerves related to nerve regeneration have not been confirmed. For this reason, we studied the origins of SP-LI fibres and CGRP-LI fibres of central area of cornea of rat by using the combined horseradish peroxidase(HRP) retrograde tracing and immunohistochemistry method. Solution of 30% HRP was injected into the corneal epithelium of central area of rat cornea under stereomicroscope. After a survival period of 2 days, the rats were perfused transcardially with 4% paraformaldehyde. The trigeminal ganglion (TG). superior cervical ganglion (SCG) and vagus ganglion (VG) were removed and frozen-sectioned serially at 40um. The sections were processed according to TMB method to show HRP labelled cells, then the sections were treated with immunohistochemistry method and examined by bright-field microscopy. The resuhs were as follows: double labelled neurons were found in the medial area of the ipsilateral TG (ophthalmic region) and concentrated at the dorsal part of the area. The CGRP-LI neurons were distributed in the peripheral part of dorsal-medial area of TG. The SP-LI neurons were scattered in the deeper pan of dorsal-medial area of TG. The shapes of SP-LI and CGRP-LI neurons are round or elliptical. There were no double labelled neurons in the SCG and VG.This experiment not only confirmed the hypothesis that the corneal SP-LI fibres originated from TG neurons but alro proved that the double labelled neuron was not found in the SCG and VG which suggested that the SP-LI fibres and CGRP-LI fibres were not come from neuorns of SCG and VG.