HPLC-ELSD法测定咳喘宁中桔梗皂苷D的含量 |
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引用本文: | 郑琰,张秀丽,崔岩. HPLC-ELSD法测定咳喘宁中桔梗皂苷D的含量[J]. 中国当代医药, 2014, 21(14): 11-13 |
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作者姓名: | 郑琰 张秀丽 崔岩 |
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作者单位: | 郑琰(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000);张秀丽(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000);崔岩(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000); |
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摘 要: | 目的 建立高效液相色谱法测定咳喘宁中桔梗皂苷D的方法.方法 采用以蒸发光散射检测器的高效液相色谱法,色谱柱为Kromasil C18(4.6 mm×250.0 mm,5μm),流动相为乙腈∶水(28∶72),流速为0.9 ml/min,ELSD漂移管温度为70℃,气体流速为3.0 SLPM/min.结果 桔梗皂苷D进样量在0.513~10.260 μg范围内呈良好线性关系(r=0.9996),平均回收率98.44%,RSD=0.88% (n=6).结论 该方法准确,可靠,重复性好,灵敏度高,可用于咳喘宁的质量控制标准.
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关 键 词: | 咳喘宁 桔梗皂苷D 高效液相色谱法-蒸发光散射检测器 |
Determination of platycodin D in Kechuanning by HPLC-ELSD method |
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Affiliation: | ZHENG Yan ZHANG Xiu-li CUI Yah (Institute for Food and Drug Control of Chaoyang City in Liaoning Province,Chaoyang 122000,China) |
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Abstract: | Objective To establish a method for determination the content of platycodin D in Kechuanning. Methods High performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD)method was used,the column was Kromasil C1s(4.6 mm×250.0 mm,5 μm),mobile phase was acetonitrile : water(28:72).The flow rate was 0.9 ml/min, the ELSD drift tube temperature was 70℃ and the gas flow rate was 3.0 SLPM/min. Results The linear range of Platy- codin D was 0.513-10.260 μg(r=0.9996).The average recovery was 98.44%,RSD=O.88%(n=6). Conclusion The method is accurate,reliable,good reproducibility,high sensitivity and can be used for the quality control of Kechuanning standard. |
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Keywords: | Kechuanning Platycodin D HPLC-ELSD |
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