健脾益气方对化疗性胃肠功能紊乱大鼠血清GAS、EGF水平的影响

目的探讨健脾益气方（JPYQ）防治大鼠化疗性胃肠功能紊乱的效果及作用机制。方法将48只大鼠按随机原则分为6组：空白对照组、化疗模型组、枸橼酸莫沙必利组（阳性药对照组）、化疗＋JPYQ高剂量组（高剂量组）、化疗＋JPYQ中剂量组（中剂量组）、化疗＋JPYQ低剂量组（低剂量组），每组8只。采用环磷酰胺制备大鼠化疗模型，在实验的第1、3、5天，空白对照组给予生理盐水腹腔注射，其余5组给予环磷酰胺注射液腹腔注射，造模后每组给予相应的药物灌胃。检测各组大鼠血清胃泌素（GAS）与表皮生长因子（EGF）的水平。结果与空白对照组比较，化疗模型组大鼠血清EGF水平明显降低，GAS含量则明显升高，差异有统计学意义（P〈0．05）；与模型组比较，JPYQ高剂量组和阳性药对照组大鼠血清EGF含量显著升高（P〈0．05），而中、低剂量组大鼠血清EGF含量差异无统计学意义（P〉0．05）；各给药组大鼠血清GAS水平与模型组及与空白对照组比较，差异无统计学意义（P〉0．05）。结论JPYQ防治化疗性胃肠功能紊乱的效果明显，其作用机制主要与影响血清EGF水平相关。

Effect of Jianpi yiqi recipe on serum GAS and EGF concentration of rats with gastrointestinal dysfunction caused by chemotherapy

Objective To investigate the effect of Jianpi yiqi recipe （JPYQ） on the gastrointestinal functional disorder in rat receiving chemotherapy. Methods Forty-eight rats were equally randomized into 6 groups,and they were blank control group,chemotherapy model group,mosapride citrate group （positive control group）,chemotherapy＋JPYQ high-dose group （high-dose group）,chemotherapy＋JPYQ middle-dose group （middle-dose group）,chemotherapy＋JPYQ low-dose group （low-dose group）,each group of 8 animals.Preparation of rat chemotherapy model was used cyclophosphamide,rats in the blank control group were given intraperitoneal injection of normal saline while the rats in the other groups were given intraperitoneal injection of cyclophosphamide on experimental day 1,3 and 5.During the modeling,the rats in the medication groups were given the corresponding medicine according to the experimental design.The levels of serum gastrin（GAS） and epidermal growth factor （EGF）in rats were determined. Results Compared with blank control group,the level of EGF in model group was significantly decreased,while the level of GAS was significantly increased （P〈0.05）. Compared with model group,the levels of EGF in high-dose JPYQ group and mosapride group were significantly increased （P〈0.05）,while the levels of EGF in middle-dose JPYQ group and low-dose JPYQ group were no significant difference （P〉0.05）.The level of GAS was insignificant between the medicated groups and model group and blank control group （P〉0.05）. Conclusion There is evident therapeutic effect of Jianpi yiqi recipe on gastrointestinal dysfunction caused by chemotherapy.And it＇s mechamism is