肥胖大鼠血糖、血脂、瘦素和下丘脑瘦素水平的变化

目的 建立营养性肥胖鼠的动物模型，观察营养性肥胖鼠中糖、脂代谢异常和胰岛素抵抗及瘦素抵抗的情况。方法 （1）高脂饮食诱导建立肥胖大鼠模型；（2）采用放射免疫法测定其血清胰岛素．瘦素和下丘脑组织的瘦素水平。结果 （1）肥胖和超重大鼠血糖、血脂、血清瘦素水平程度显著升高；（2）肥胖组和超重组大鼠下丘脑组织的瘦素含量低于正常组；（3）大鼠的体重、TG、HOMA—IR与血清瘦素水平呈显著正相关，而与下丘脑瘦素水平呈负相关。结论 肥胖鼠体内存在糖、脂代谢异常和胰岛素抵抗，同时还存在瘦素抵抗。这些代谢紊乱对肥胖的发生具有重要意义。

Changes of blood glucose,serum lipids,leptin and the levels of hypothalamic leptin in diet-induced obese rats

Objective To investigate both the metabolic disorders of glucose,lipids,insulin and leptin,and correlation between insulin resistance and leptin resistance and obesity.Methods Serum lipids TC,TG and blood glucose were measured after the experiment.Serum insulin,leptin and the levels of leptin in hypothalamus were detected by radioimmunoassay.Results(1) Both the levels of blood glucose,serum TC,TG,insulin and leptin,and the degree of insulin resistance(HOMA-IR) in obese and overweight group were higher than those of normal controls.(2) The levels of leptin in hypothalamus in obese and overweight group were lower significantly.(3) There was positive correlation between the serum leptin and the the levels of serum TG,TC and HOMA-IR respectively.(4) There was negative correlation between the level of hypothalamic leptin and of serum TG and HOMA-IR.Conclusion Metabolic disorders of glucose,lipids,insulin resistance and leptin resistance are observed in diet-induced obese rats and they play an important roles in pathological genesis of obesity.