| 抗凝血酶Ⅲ浓缩物制备及临床应用的研究 一、抗凝血酶Ⅲ的分离与纯化 |
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| 引用本文： | 刘文方,刘晓宇,余蓉.抗凝血酶Ⅲ浓缩物制备及临床应用的研究 一、抗凝血酶Ⅲ的分离与纯化[J].中国输血杂志,1988(3). |
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| 作者姓名： | 刘文方 刘晓宇 余蓉 |
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| 作者单位： | 中国医学科学院输血研究所，中国医学科学院输血研究所，中国医学科学院输血研究所 |
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| 摘 要： | 本文报道用肝素-Sepharose 4B凝胶批式亲和吸附法,成功地从血浆中分离、纯化出抗凝血酶Ⅲ(AT-Ⅲ)。该法适于批量制备,整个分离过程仅需3天。从20升血浆中获得的AT-Ⅲ活性回收率为22.4％—42.3％,进一步纯化后的AT-Ⅲ活性及抗原回收率分别为26.3％和20.3％,其比活性为9.9U/mg蛋白,在聚丙烯酰胺凝胶电泳图谱上显示单一的区带;其纯度、生物活性及回收率接近国外有关报道。
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A Study on Preparation and Clinical Application of Antithrombin Ⅲ Concentrate Ⅰ. Isolation and Purification of Antithrombin Ⅲ |
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| Abstract: | A batch affinily adsorption method with heparin-sepharose 4B gel has been described for preparation and purification of antithrombin Ⅲ from plasma. The me- thod is suitable for batch preparation. The processes of each batch are completed only in 3 days. The recovery of activity was 22.4-42.3% from 20 liters of plasma. By fur- ther purification, the recovery of activity and antigen was 26.3% and 20.3% respec- tively, the specific activity was 9.9U/mg. The purified AT-Ⅲ showed a single band on disc gel electrophoresis, and the levels of purity, biologic activity and the recov- ery of activity are similar to those of for- eign reports. |
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