HLA-DQA1基因与广西地区2型糖尿病关联性的研究

目的:探讨2型糖尿病(DM)及其并发症与HLA的相关情况.方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)法对72名广西汉族2型糖尿病(DM)及46名汉族正常对照的HLA-DQA1位点进行基因分型.结果:2型DM组DQA1*0401等位基因频率明显低于正常对照组,DOA1*0104等位基因频率明显高于正常对照组,2型DM并肾病组和并高血压组DQA1*0501等位基因频率分别低于无肾病组和无高血压组.结论:广西汉族2型DM及其并发症与HLA有关联;HLA-DQA1*0401可能为2型DM的保护基因,HLA-DQA1*0104可能为2型DM的易感基因;HLA-DQA1*0501对2型DM并肾病、高血压具有保护作用.

RELATIONSHIP BETWEEN HLA-DQA1 GENE WITH TYPE 2 DIABETES IN GUANGXI

To evaluate the relationship between HLA a nd type 2 diabetes and it's complicationsMethods:HLADQA1 alleles in 72 patie nts and 46 controls were investigated by Polymerase Chain ReactionSequence Spe cific Primers (PCR-SSP) techniqueResults:The frequency of H LA-DQA1*0401 was significantly in decreased in the patients of type 2 diabetes compared with healthy control, and the frequency of HLA-DQA1*0104 was significa ntly increased in the patientsThe frequency of HLA-DQA1*0501 was lower in dia betic nephropathyThe HLA-DQA1*0501 alleles in diabetic hypertension group was significantly decreasedConclusion:HLA-DQA1 gene was associ ated with type 2 diabetes and it's complications, and HLA-DQA1*0401 might prote ct individuals from development of type 2 diabetes, while HLADQA1*0104 was sho wn to be a susceptible geneHLA-DQA1*0501 might protect individuals from nephr opathy and hypertension