Distribution of axons exhibiting both enkephalin- and serotonin-like immunoreactivities in the lumbar cord segments: an immunohistochemical study in the cat

Axons exhibiting both enkephalin- and serotonin-like immunoreactivities were observed by the double immunofluorescence method in the lumbar cord segments of the cat. Double-labeled axons were seen most frequently in laminae I. IIa and the lateral part of lamina V. They were also distributed in other parts of the dorsal horn and lamina X (especially in the dorsal part), but rarely found in laminae VII, VIII and IX. After cervical hemicordotomy the vast majority of double-labeled axons disappeared from the spinal gray ipsilateral to the lesion.