金雀异黄素对兔角膜碱烧伤新生血管的抑制作用

目的:探讨金雀异黄素(genistein,Gen)对兔角膜新生血管(corneal neovascularization,CNV)的抑制作用。方法:取新西兰大白兔13只,不造模1只观察药物对眼表前房等组织的不良反应。其余12只24眼采用浸润1mol/LNaOH滤纸片贴敷角膜中心60s,诱导碱烧伤CNV形成,左眼用二甲亚砜(dimethylsulfoxide,DMSO)溶液,右眼用Gen的DMSO溶液点眼。碱烧伤后3,7,14,21d测量角膜CNV面积,处死相应组别的动物取眼球做切片HE染色和免疫组织化学染色观察角膜和前房等情况。结果:兔角膜碱烧伤后CNV存在生长和消退的病理变化,Gen+DMSO组角膜CNV面积与DMSO组相比有明显的统计学差异性(P<0.01)。结论:Gen对于兔角膜碱烧伤模型的CNV有明显的抑制作用。

Inhibition of experimental alkali-induced corneal neovascularization in rabbits by using genistein

AIM:To evaluate the effect of genistein (Gen) administration on corneal Alkali-induced corneal neovas-cularization (CNV) in rabbits. ·METHODS:One of 13 normal New Zealand white rabbits was used to observe the drug adverse reactions in the anterior chamber and other organizations. The remaining 12 rabbits (24 eyes) were used of 1mol/L NaOH infiltration applicated of filter paper on the center cornea for 60 seconds,induced by alkali burn in the formation of CNV,then were randomly divided into 4 groups as 3,7,14 days and 21 days. Immediately after alkali burn on eyes,rabbits were administered by dimethyl sulfoxide (DMSO) solution on the left eyes (control) and Gen soluted by DMSO on the right eyes. Digital photographs of the cornea were taken and analyzed to determine the area of the cornea covered by CNV on the day of 3,7,14 days and 21days after alkali burn. In addition,HE staining and immunohistochemical analysis was used to determine vascular endothelial growth factor (VEGF) expression in corneal tissue after the rabbits were excuted. ·RESULTS:Analysis of digital photographs showed that there was less CNV Gen-treated eyes than in controls (P<0.01,compired t test). In addition,there was less staining for VEGF in corneas from Gen-treated eyes than in control eyes. Gen solution drops were not associated with any complications during observation. ·CONCLUSION:Genistein administration decreased alkali burn corneal neovascularization in rabbits.