| 三组中药的电泳指纹图谱研究 |
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| 引用本文： | 石俊英,卢燕,徐凌川,李渤文.三组中药的电泳指纹图谱研究[J].中药材,2002,25(12):864-868. |
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| 作者姓名： | 石俊英 卢燕 徐凌川 李渤文 |
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| 作者单位： | 山东中医药大学中药学院,济南250014 |
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| 摘 要： | 目的:利用电泳指纹图谱鉴别二组中药。方法:对三组不同种或不同农家品种的中药分别进行蛋白质、过氧化物酶(POD)同丁酶及酯酶(ES)同工酶三种聚丙烯酰胺凝胶电泳分析,并建立相应的指纹图谱。结果:各样品组的三种电泳指纹图谱至少有一种存在显著差异,可准确区分该组的所有样品。结论:选择适当的电泳方法可准确鉴别不同种甚至不同农家品种的中药。
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| 关 键 词： | 中药 电泳 指纹图谱 聚丙烯酰胺凝胶 |
| 修稿时间： | 2002年7月24日 |
Study on the Electrophoresis Finger-prints of Three Groups of Traditional Chinese Drugs |
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| Shi Junying.Lu Yan.Xu Lingchuan,Li Bowen.Study on the Electrophoresis Finger-prints of Three Groups of Traditional Chinese Drugs[J].Jorunal of Chinese Medicinal Materials,2002,25(12):864-868. |
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| Authors: | Shi JunyingLu YanXu Lingchuan Li Bowen |
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| Institution: | Traditional Chinese Medicinal College, Shandong University of Traditional Chinese Medicine, Jinan 250014. |
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| Abstract: | Objective:To identify three groups of traditional Chinese drugs by electrophoresis finger-prints. Methods:The 3 groups of drugs of different species or varieties were analyzed by protein,peroxidase(POD)isozyme and esterase(ES)isozyme polyacrylamide gel electrophoresis, respectively. And their finger-prints were established. Results: Not less than one kind of electrophoresis finger-prints has significant difference between the drugs of each group, which can be used to distinguish all the species in this group. Conclusions: Different species even different varieties of traditional Chinese drugs can be identified accurately with proper electrophoresis finger-prints. |
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| Keywords: | Traditional Chinese drug Electrophoresis Finger- prints Polyaciylamide gel |
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