APP17肽对糖尿病大鼠海马线粒体膜电位的影响

应用流式细胞仪分别观察糖尿病模型组、糖尿病APP1 7肽治疗组和正常对照组大鼠海马线粒体膜电位和凋亡率。结果 :糖尿病组大鼠海马线粒体膜电位明显下降 (P <0 .0 1 ) ,凋亡发生率增加 (P <0 .0 1 ) ;APP1 7肽治疗组与糖尿病组相比海马线粒体膜电位增高 (P <0 .0 5 ) ,凋亡发生率降低 (P <0 .0 5 ) ,与正常对照组比较无显著性差异。结果提示 :海马线粒体膜电位下降和细胞的凋亡可能参与糖尿病脑病的发生和发展 ;APP1 7肽具有改善这一病理过程的作用

Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats

The objectives were to investigate the role of the alteration of mitochondria transmembrane potentials in apoptosis in hippocampal neurons of STZ DM rats and the possible effect of APP 17 peptide on it. DM models were established by injection of streptozotocin intraperitoneal, the APP 17 peptide treated group were injected with APP 17 peptide three times a week, whereas the control and DM groups were given saline. Single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the percentage of cell in apoptosis were measured by means of flow cytometry (FCM) at the 11th week when the animals were sacrificed. The neurons in hippocampus of DM group had lower mitochondria transmembrane potentials and higher apoptotic rate than those of the control group. APP 17 peptide could significantly blocked the mitochondria transmembrane potentials collapse and apoptosis. Decreased mitochondria transmembrane potential and related apoptosis may contribute to the mechanism of diabetic encephalopathy. APP 17 peptide palys an anti apoptosis role in it.