| 小干扰RNA的设计及原理 |
| |
| 引用本文： | 张镁,刘苏虎. 小干扰RNA的设计及原理[J]. 医学分子生物学杂志, 2005, 2(3): 240-242 |
| |
| 作者姓名： | 张镁 刘苏虎 |
| |
| 作者单位： | 西安交通大学第二医院病理科,西安市,710004;西安交通大学第二医院血液科,西安市,710004 |
| |
| 摘 要： | RNA干扰技术在人类基因功能研究、药物靶点鉴定等方面备受青睐,并且具有潜在的临床应用价值。但只有设计合理的小干扰RNA (siRNA)才能高效、特异地抑制靶基因表达。本文总结了siRNA序列的一般特点以及设计siRNA的方法和相应原理,并介绍了如何利用实验手段,验证RNA干扰的有效性及特异性。从而促进RNA干扰技术的广泛开展。
|
| 关 键 词： | RNA干扰 小干扰RNA 序列设计 |
| 修稿时间： | 2004-11-25 |
Design of siRNA for Efficient and Specific Inhibition of Target Gene Expression in Mammalian Cells |
| |
| ZHANG Mei,Liu Suhu. Design of siRNA for Efficient and Specific Inhibition of Target Gene Expression in Mammalian Cells[J]. Journal of Medical Molecular Biology, 2005, 2(3): 240-242 |
| |
| Authors: | ZHANG Mei Liu Suhu |
| |
| Affiliation: | ZHANG Mei~1,LIU Suhu~2 1 Department of Pathology,the Second Hospital,Xi'an Jiaotong University,Xi'an,710004,China 2 Department of Hematology,the Second Hospital,Xi'an Jiaotong University,Xi'an,710004,China |
| |
| Abstract: | RNA interference technique has been revolutionizing functional genomics, drug target identification and validation. And they are potentially valuable in clinical practice. Only well-designed siRNA can specifically and efficiently inhibit the expression of the target genes. This article reviews the features of siRNA sequences, principles and mechanisms of siRNA design, and the experimental strategies, testing of the efficiency and specificity of RNA interference, to provide information for the wider application of RNA interference technique. |
| |
| Keywords: | RNA interference siRNA sequence design |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
