聚合酶链反应诊断结核性腹膜炎的评价

目的评价聚合酶链反应（ＰＣＲ）方法对结核性腹膜炎的诊断价值。方法用ＰＣＲ技术检测３０例结核腹水中结核分支杆菌ＤＮＡ，并与腹水涂片抗酸染色及酶联免疫吸附试验（ＥＬＩＳＡ）检测腹水中抗ＰＰＤ抗体进行比较。结果ＰＣＲ的阳性率为６０％，特异性９４．４％；ＥＬＩＳＡ法阳性率６３．３％，特异性７２．２％；涂片镜检均为阴性。结论ＰＣＲ在诊断结核性腹膜炎具有较高的敏感性和特异性，优于ＥＬＩＳＡ法及涂片镜检，如与ＥＬＩＳＡ技术结合可进一步提高检测的敏感性和特异性。

Evaluation on the diagnostic value of the Polymerase Chain Reaction assayin the detection of uberculous peritonitis

Objective To evaluate the diagnostic value of the PCR assay in the detection of tuberculous peritonitis. Method Thirty cases of tuberculous ascites were tested for the DNA of mycobacteria tuberculosis with the PCR technique.The outcome was compaired with those by the acid fast staining on smear and the enzymelinked immunosorbent assay(ELISA). Results With the PCR technique, the positivity was 60% and the peculiarity was 94.4%, while with the ELISA, the posilivity and peculiarity were 63.3% and 72.2% respectively. Both were the acid fast staining. Conclutions The PCR assay has a better peculiarity and a fairly good sensitivity in the detection of tuberculous peritonitis and therefore is superior to the acid fast staining and ELISA, If the PCR and the ELISA are employed in combination. the sensitivity and the peculiarity of the test might be well raised up.