| 肿瘤坏死因子α致肝细胞凋亡效应及其机制的研究 |
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| 引用本文： | Weiqing Song Weihong Lu Huabo Chen Huahui Li Jianmin Wu. 肿瘤坏死因子α致肝细胞凋亡效应及其机制的研究[J]. 中德临床肿瘤学杂志, 2008, 7(2): 72-74. DOI: 10.1007/s10330-007-0122-4 |
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| 作者姓名： | Weiqing Song Weihong Lu Huabo Chen Huahui Li Jianmin Wu |
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| 作者单位： | [1]Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China [2]Qingdao Municipal Hospital, Qingdao 266011, China |
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| 摘 要： | Objective: To investigate the apoptosis effect of TNF-a on HepG2 cells and its mechanism in vitro.Methods: HepG2 cells were treated with TNF-α(400 U/mL).HepG2 cells treated with TNF-αfor 24 h and apoptosis was proved by DNA fragments on gel electrophoresis.Fluorescent quantitative real-time PCR was used to detect Fas and FasL expression.HepG2 cells treated by TNF-αwere co-cultivated with normal HepG2 cells.Apoptosis of HepG2 cells was determined by the method of FACS.Results: After 24 h TNF-αtreatment, DNA was collapsed into fragments to form DNA ladder in gel electrophoresis; FasL expression increases and induces HepG2 cells apoptosis.By FACS, 98.4% of the co-cultivated cells were apoptosis, but 16.5% cells in control group were apoptosis.Conclusion: TNF-αcan induce apoptosis of HepG2 cells in vitro.FasL expression on TNF-αpre-treated HepG2 cells increased and these cells can lead normal HepG2 cells to apoptosis.This may attribute to the cure of virus hepatitis and hepatoma.
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| 关 键 词： | TNF-α |
| 文章编号： | 10.1007/s10330-007-0122-4 |
| 收稿时间： | 2007-07-15 |
| 修稿时间： | 2007-09-17 |
Studies of hepatocytes apoptosis induced by TNF-α and its mechanism |
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| Weiqing Song,Weihong Lu,Huabo Chen,Huahui Li,Jianmin Wu. Studies of hepatocytes apoptosis induced by TNF-α and its mechanism[J]. The Chinese-German Journal of Clinical Oncology, 2008, 7(2): 72-74. DOI: 10.1007/s10330-007-0122-4 |
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| Authors: | Weiqing Song Weihong Lu Huabo Chen Huahui Li Jianmin Wu |
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| Affiliation: | (1) Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China;(2) Qingdao Municipal Hospital, Qingdao, 266011, China |
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| Abstract: | Objective To investigate the apoptosis effect of TNF-α on HepG2 cells and its mechanism in vitro. Methods HepG2 cells were treated with TNF-α (400 U/mL). HepG2 cells treated with TNF-α for 24 h and apoptosis was proved by DNA fragments on gel electrophoresis. Fluorescent quantitative real-time PCR was used to detect Fas and FasL expression. HepG2 cells treated by TNF-α were co-cultivated with normal HepG2 cells. Apoptosis of HepG2 cells was determined by the method of FACS. Results After 24 h TNF-α treatment, DNA was collapsed into fragments to form DNA ladder in gel electrophoresis; FasL expression increases and induces HepG2 cells apoptosis. By FACS, 98.4% of the co-cultivated cells were apoptosis, but 16.5% cells in control group were apoptosis. Conclusion TNF-α can induce apoptosis of HepG2 cells in vitro. FasL expression on TNF-α pre-treated HepG2 cells increased and these cells can lead normal HepG2 cells to apoptosis. This may attribute to the cure of virus hepatitis and hepatoma. |
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| Keywords: | apoptosis Fas/FasL hepatocyte |
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