| 珍稀中草药金线莲的RAPD研究 |
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| 引用本文： | 胡珊梅,张启国,袁文杰,周涵韬,周兴旺,陈鹭真. 珍稀中草药金线莲的RAPD研究[J]. 中草药, 2000, 31(12): 944-946 |
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| 作者姓名： | 胡珊梅 张启国 袁文杰 周涵韬 周兴旺 陈鹭真 |
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| 作者单位： | 1. 厦门市药品检验所,厦门,361012
2. 厦门大学生命科学学院 |
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| 基金项目： | 厦门市卫生局医学科学技术进步基金科研资助项目 |
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| 摘 要： | 为了鉴别金线莲的品种，探索同属植物之间的亲缘关系及同种植物不同产地的遗传变异，并制定正品金线莲的DNA指纹图谱，采用随机扩增多态DNA(RAPD)技术对兰科开唇兰属植物花叶开唇兰Anoectochilus roxburghii(Wall．)．Lindl．、台湾开唇兰A.formosanus Hay．进行了鉴定，结果选择的7个引物对3个品种共扩增出98个位点，其中3个引物为高特异性引物。结论 RAPD技术不仅能鉴别种间差异，而且能揭示同种不同产地植物的遗传变异。
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| 关 键 词： | 中草药 金线莲 RAPD技术 遗传变异 药物研究 |
| 收稿时间： | 2000-03-12 |
Studies on Valuable and Rare Chinese Herbs Anoectochilu s roxburghii by DNA Fingerprintings Using RAPD |
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| Hu Shanmei,Zhang Qiguo,Yuan Wenjie,Zhou Hantao,Zhou Xingwang and Chen Luzhen. Studies on Valuable and Rare Chinese Herbs Anoectochilu s roxburghii by DNA Fingerprintings Using RAPD[J]. Chinese Traditional and Herbal Drugs, 2000, 31(12): 944-946 |
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| Authors: | Hu Shanmei Zhang Qiguo Yuan Wenjie Zhou Hantao Zhou Xingwang Chen Luzhen |
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| Affiliation: | Xiamen 361012 |
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| Abstract: | In order to explore the consanguinity of plants of genus Anoectochilus Bl. (Orchidaceae), and to dis tinguish Anoectochilus roxburghii (Wall.) Lindl. from two diffe r ent localities, Fujian and Guangxi Province, and A. formosanys Hay as to their genetic variance, DNA fingerprintings were analysed with the ai d o f RAPD. Results showed that 7 primers amplified a total of 98 sites for the 3 s pecies. 3 of the 7 primers were highly specific for genuine A. roxburgh i i. Thus, the 3 species could be distinguished according to the bonding pattern of their amplified DNA on agarose gel. It was concluded that the method may be of value for the authentication of different species and same species of Anoectochilus Bl. from different localities |
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| Keywords: | RAPD marker Anoectachilus roxburchii (Wall.) Lindl. (Jin Xian Lian) genetic variation |
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