螺旋体与牙龈卟啉单胞菌在口臭形成中的作用

目的　评价螺旋体和牙龈卟啉单胞菌各自在产生挥发性硫化物 (volatilesulfurcompounds,VSCs) ,形成口臭中的作用。 方法　对 117例牙周炎患者 ,用鼻闻法评价口臭值(organolepticrating ,OR)后分成口臭组 85例 ,无口臭组 32例 ,用口臭测量仪测量口气中VSCs水平 ,刚果红负性染色法检测舌苔和龈下菌斑中螺旋体的百分比 (S % ) ,间接免疫荧光法检测牙龈卟啉单胞菌 (Porphyromonusgingivalis ,Pg)。口臭组 4 6例在牙周基础治疗后重复以上检查。结果　口臭组探诊深度 (probingdepth ,PD) <4mm位点S %明显高于无口臭组 (P <0 0 1) ,而舌苔与PD≥ 4mm位点的S %、舌苔与龈下菌斑中的Pg均无差异。口臭组OR、VSCs水平与舌苔及PD <4mm位点S %正相关 ,与PD≥ 4mm位点的S %无关 ;OR值与PD≥ 4mm位点的Pg呈正相关 (P <0 0 5 ) ,与舌苔及PD <4mm位点的Pg无关 ,而VSCs与 3个部位的Pg均无关系 ;牙周治疗后OR和VSCs水平明显降低 ,舌苔中的S %、龈下菌斑中的S %及Pg均明显下降 (P <0 0 1)。结论　螺旋体是与VSCs及口臭的产生有关的口腔微生物之一 ,牙周治疗可以改善口臭

Effect of spirochetes and Porphyromonus gingivalis on oral malodor

OBJECTIVE: To assess the effect of Spirochetes and Porphyromonus gingivalis (Pg) on the production of volatile sulfur compounds (VSCs) and oral malodor, respectively. METHODS: 117 systemically healthy periodontitis patients were divided into two groups according to organoleptic rating (OR) by organoleptic method: 85 subjects with oral malodor (halitosis group, HG) and 32 cases without oral malodor (no-halitosis group, NHG). VSCs measurements were made with halimeter. Subgingival plaques and tongue coating were taken to calculate the proportion of Spirochetes (with 2% Congo Red Smears, S%) and the amounts of Pg (with indirect immunofluorescence method). The examination will be repeated in 46 HG cases after periodontal therapy. RESULTS: S% in PD < 4 mm sites of HG was significantly higher than that in NHG (P < 0.01). S% in PD >or= 4 mm sites and tongue coating, Pg in all three sites were not different between two groups. In HG, OR and VSCs levels were correlated with S% in tongue coating (P < 0.01, P < 0.05) and PD < 4 mm sites (P < 0.05), and not correlated with S% in PD >or= 4 mm sites. OR was related to Pg in PD >or= 4 mm sites (P < 0.05) and there was no relationship between OR and Pg in tongue coating and PD < 4 mm sites. VSCs levels was not correlated with Pg in all three sites. S% in tongue coating and subgingival plaque, Pg in subgingival plaque, OR and VSCs levels were significantly reduced after periodontal therapy (P < 0.01). CONCLUSIONS: The direct relationship of Spirochetes and Pg respectively to bad breath was not found obviously. But Spirochetes is one of the microorganism on oral malodor. Periodontal therapy can improve oral malodor.