| 糖皮质激素治疗三氯乙烯药疹样皮炎患者外周血淋巴细胞基因表达谱研究 |
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| 引用本文： | 刘玥,梁丽燕,马文丽,越飞,郑文岭.糖皮质激素治疗三氯乙烯药疹样皮炎患者外周血淋巴细胞基因表达谱研究[J].中国现代医学杂志,2006,16(22):3361-3364,3367. |
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| 作者姓名： | 刘玥 梁丽燕 马文丽 越飞 郑文岭 |
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| 作者单位： | 1. 南方医科大学基因工程研究所,广东,广州,510515
2. 广东省职业病防治院,广东,广州,510300 |
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| 基金项目： | 广东省医学科学技术研究基金 |
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| 摘 要： | 目的探讨三氯乙烯药疹样皮炎在糖皮质激素治疗前后的基因表达谱变化。方法从1例三氯乙烯药疹样皮炎患者糖皮质激素处理前后的外周血中分离淋巴细胞，提取总KNA，合成cKNA并分别用Cy3和Cy5标记，与Agilent Human 1B寡核苷酸基因芯片杂交，研究基因表达谱变化。结果在检测的20173个基因中，筛选出共同差异表达基因1016个，其中上调618个，下调398个，和免疫与防御相关基因52个。结论和免疫与防御相关的52个基因可能为三氯乙烯药疹样皮炎的疾病相关基因。
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| 关 键 词： | 三氯乙烯药疹样皮炎 基因表达谱 基因芯片 |
| 文章编号： | 1005-8982（2006）22-3361-04 |
| 收稿时间： | 2006-12-02 |
| 修稿时间： | 2006-12-02 |
Gene expression research of peripheral blood lymphocytes of DMLT patients treated with Glucocorticoid |
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| LIU Yue,LIANG Li-Yan,MA Wen-Li,YUE Fei,ZHENG Wen-Ling.Gene expression research of peripheral blood lymphocytes of DMLT patients treated with Glucocorticoid[J].China Journal of Modern Medicine,2006,16(22):3361-3364,3367. |
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| Authors: | LIU Yue LIANG Li-Yan MA Wen-Li YUE Fei ZHENG Wen-Ling |
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| Institution: | 1.Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong 510515 P.R China; 2.Hospital for Occupational Diseases Control of Guangdong Province, Guangzhou, Guangdong 510300 P.R China |
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| Abstract: | Objective To study the gene expression of the Trichloroethylene induced medicamentosa-like dermatitis (DMLT) before and after Glucocorticoid treated. Methods The total RNAs were extracted and purified from peripheral blood lymphocytes of one Trichloroethylene induced medicamentosa-like dermatitis patient before and after Glucocorticoid treated to synthesize cRNA. Then cRNA were labeled to hybridize with the Agilent human 1B 60mer oligonucleotide microarray. Results In the 20173 genes tested, 1016 genes were found expressed differentially, of these genes, 618 genes were up-regulated while 398 genes were down-regulated, and 52 genes were related with immunity and defense. Conclusion 52 genes related with immunity and defense may have a correlation with DMLT. |
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| Keywords: | trichloroethylene induced medicamentosa-like dermatitis gene expression profiling microarray |
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