合体滋养细胞微粒对人脐静脉内皮细胞增殖和凋亡的影响

目的:检测合体滋养细胞微粒(STBM)对人脐静脉内皮细胞(HUVECs)增殖和凋亡的影响,探讨子痫前期可能的病因和发病机制。方法:选取2009年6月至10月在上海交通大学附属第六人民医院住院行选择性剖宫产的4例正常健康孕妇的胎盘,机械法体外制备STBM。STBM与HUVECs共培养,MTT法和流式细胞仪检测HUVECs的增殖和凋亡情况。结果:(1)STBM作用12h后,HUVECs悬浮细胞数增加,48h后可见大量悬浮细胞,细胞生存受到明显抑制;(2)30、90、150mg/L STBM作用48h后,内皮细胞增殖抑制率分别为(55.58±2.40)%、(67.94±1.93)%、(86.37±1.59)%,组间差异显著(P<0.01),呈剂量依赖性。(2)30、90、150mg/L STBM作用24h后,内皮细胞凋亡率分别为(17.39±1.11)%、(22.13±1.02)%、(30.42±1.98)%,组间差异显著(P<0.01),呈剂量依赖性。结论:STBM可抑制HUVECs细胞增殖,诱导细胞凋亡,提示其可能是子痫前期内皮细胞功能紊乱的原因。

Effects of syncytiotrophoblast microparticles on proliferation and apoptosis of human umbilical vein endothelial cells

Objective:To provide a basis for the pathogenesis of preeclampsia by investigating whether syncytiotrophoblast microparticles(STBM) could cause the maternal vascular endothelial dysfunction.Methods:Placentas were obtained after elective cesarean section from four uncomplicated pregnancies from June 2009 to Oct.2009 in the Sixth Affiliated Hospital of Shanghai Jiaotong University.Different concentrations of STBM and RBC ghost particles were cocultured with human umbilical vein endothelial cells.Proliferation and apoptosis rates of human umbilical vein endothelial cells were measured by MTT and flow cytometry.Results:(1)When human umbilical vein endothelial cells were incubated together with STBM preparation,the cell monolayer was disrupted,leading to cell detachment.(2)After 48h of incubation,large amounts of human umbilical vein endothelial cells detached and the number of cells were significantly reduced.STBM at 30,90 and 150mg/L respectively caused an average of(55.58±2.40)%,(67.94±1.93)% and(86.37±1.59)% inhibition of proliferation.There were significant differences among the groups(P<0.01).STBM preparations inhibited human umbilical vein endothelial cells proliferation in a dose-dependent manner.(3)After 24h,the percentage of apoptosis was(17.39±1.11)%,(22.13±1.02)%,(30.42±1.98)% for STBM at 30,90,150mg/L,respectively.With the raised concentration of STBM,apoptosis rate of human umbilical vein endothelial cells significantly increased(P<0.01).Conclusion:STBM inhibits the proliferation and induces cells apoptosis of human umbilical vein endothelial cells,which may contribute to the endothelial dysfunction underlying the maternal syndrome of preeclampsia.