HtrA1在子痫前期孕妇血清、胎盘组织中的表达及意义

目的:探讨HtrA1(high temperature requirement A1)在子痫前期孕妇血清、胎盘组织中的表达及意义。方法:HtrA1属分泌型蛋白,可采用酶联免疫吸附法(ELISA)测定同期住院孕妇的空腹血清HtrA1浓度,其中正常晚期妊娠30例、轻度子痫前期25例、重度子痫前期20例;同时应用免疫组化SABC法检测胎盘组织中HtrA1的表达。结果:正常晚期妊娠、轻度子痫前期及重度子痫前期孕妇血清HtrA1浓度分别为(160.63±32.52)pg/ml、(210.82±32.52)pg/ml及(233.68±38.61)pg/ml,组间差异显著(P<0.05);正常晚期妊娠、轻度子痫前期及重度子痫前期孕妇胎盘组织中HtrA的平均灰度值分别为156.03±5.07、149.89±2.69及140.05±4.96,组间差异显著(P<0.05)。结论:随着病情加重,孕妇血清及胎盘组织中HtrA1的表达显著增高。HtrA1与子痫前期的发生、发展有关。

The significance and expression of high temperature requirement A1 (HtrA1) in serum and placenta of pre-eclampsia

Objective:To investigate the expression and significance of high temperature requirement A1(HtrA1) in serum and placenta of pre-eclampsia.Methods:HtrA1 was secretory protein.30 normal pregnant women(control group) and 45 women with hypertensive disorder-complicating pregnancy(study group)(including 25 cases of mild preeclampsia and 20 cases of severe preeclampsia) were recruited at the same time.Measured the concentration of serum HtrA1 by enzyme-linked immunosorbent assay(ELISA),as while detected the expressions of HtrA1 in placenta by immunohistochemical SABC methods.Results:The concentration of serum HtrA1 was significantly higher in the study group than that in control group(P<0.05).The concentrations were(160.63±32.52)pg/ml,(210.82±32.52)pg/ml and(233.68±38.61)pg/ml,respectively.The same result was detected in the level of HtrA1 expression(the value of grey were 156.03±5.07,149.89±2.69 and 140.05±4.96)(P<0.05).Conclusion:With the development of pre-eclampsia,the expression of HtrA1 was increased.HtrA1 may participate in the occurrence and development of pre-eclampsia.