Notch1蛋白在正常妊娠早中晚期胎盘绒毛中的表达变化及其意义

目的:检测Notch1蛋白在正常妊娠早、中、晚期胎盘绒毛中的表达水平变化,探讨Notch1在胎盘形成中的作用及意义。方法:采用免疫组织化学SP法、Real-timePCR、Western blot分别检测20例正常早期绒毛组织、15例中期妊娠和20例晚期妊娠胎盘组织中Notch1 mRNA及蛋白的表达水平。结果:Notch1蛋白在正常早、中、晚期妊娠过程中均有不同程度的表达,随着妊娠的进展其表达水平呈下调趋势,但无统计学差异(P>0.05)。正常早期绒毛组织中Notch1 mRNA的表达水平显著高于妊娠中、晚期胎盘组织(P<0.05)。结论:Notch1蛋白可能参与正常妊娠早期绒毛的分化及胎盘的形成过程,有利于维持妊娠的正常发展。

Expression of Notch1 protein in villi and placental tissue during the normol pregnancy

Objective:To detect the expression of Notch1 protein in villi and placental tissue respectively during the normol frist trimester,midtrimester and late pregnancy,and to investigate its role in the placenta formation process.Methods:To detect the expression of Notch1 in normal villi tissue of the first trimester,normal placental tissue of the midtrimester and late pregnancy by immunohistochemical SP,RT-PCR and Western blot.Villus and placent were obtained repectively from 20 women with normal first trimester pregnancy,15 with midtrimester pregnancy and 20 with the late pregnancy.Results:The expression level of Notch1 protein was down-regulation during the three different trimester.Expression of Notch1 mRNA in villi tissue of the normal first trimester was significantly high in comparison to normal midtrimester and later pregnancy(P<0.05).Conclusion:Notch1 protein may be involved villi differentiation in first trimester and placenta formation process,and benefical to normal successful pregnancy.