骨髓间充质干细胞在大鼠小肠缺血-再灌注损伤的保护作用

目的:探讨骨髓间充质干细胞(MSC)对大鼠小肠缺血.再灌注(I/R)损伤时肠屏障的保护作用.方法:Ficoll法分离培养大鼠的MSC.36只大鼠随机分为对照组和治疗组,每组18只.治疗组在建模后60 min,通过尾静脉将1 X 10<'6>/ml的MSC注射至大鼠体内,对照组注射等量的等渗盐水.于输注后6、24和72 ...

Protective effects of bone marrow mesenchymal stem cells on small intestine ischemia/reperfusion injury in rats

Objective: To investigate the prevention of bone marrow mesenchymalstem cells(MSC) transplantation against small intestine ischemia/reperfusion injury in rats.Method: Rat MSC was isolated and cultured with the Ficoll method.Thirty-six Sprague-Dawley(SD) rats were randomly divided into control group(n=18),treatment group(n=18).In treatment group,MSC was infused by caudal vein with each rat 1×106 cells 60 min after I/R I model induction while NS was used in the control group.Serum IL-6,IL-10,TNF-α,MDA levels were detected.Pathological changes in intetinal tissue were examined.Results: Serum levels of IL-6 and TNF-α in treatment group were significantly lower than in control group at 6 h and 24 h(P<0.05),but IL-10 was higher(P<0.05).There were significant differences between the two groups at 6 h,24 h and 72 h of serum MDA level(P<0.05) and at 24 h of serum SOD level(P<0.01).DAPI positive cells could be seen in intestinal tissue of treatment group rats with fluorescent microscope.Pathological and immunohistochemical results showed that the necrosis of enterocyte was more gently at 6 h and 24 h and the generation was obvious at 24 h and 72 h in treatment group(P<0.05).Conclusion: Transplanted BMSC can transfer to and settle at injuried intestine and shows the beneficial effects on intestinal I/RI.MSC have the ability to regular inflammatory response induced by intestinal I/RI.