联合检测AKA、抗-CCP和RF在类风湿性关节炎诊断中的价值

目的探讨联合检测抗角蛋白抗体(AKA)、环瓜氨酸肽抗体(抗-CCP)和类风湿因子(RF)诊断类风湿关节炎(RA)的临床意义。方法检测187例RA患者和30例非RA患者的AKA、抗-CCP和RF。应用间接免疫荧光法测定AKA,应用酶联免疫吸附试验(ELISA)测定抗-CCP,应用速率散射比浊法检测RF。结果 187例RA患者血清中,AKA的灵敏度为58%,特异性为93%;抗-CCP的灵敏度为73%,特异性为95%;RF的灵敏度为67%,特异性为77%。三项联合检测灵敏度为97%,特异性为25%。结论 AKA、抗-CCP和RF三项联合检测可提高诊断RA的敏感性。

Value of combined detection of AKA, anti - CCP and RF in diagnosis of rheumatoid arthritis

Objective To investigate the clinical significance of combined detection of anti-keratin antibody （AKA） ,anti-cyclic citrullinated peptide（anti-CCP） antibody and rheumatoid factor（RF） in thediagnosis of rheuma- toid arthritis（RA）. Methods AKA,ant-CCP and RF were detected in 187 cases of RA patients and 30 non-RA pa- tients. The indirect immunofluoreseence assay was adopted to detect AKA,the enzyme linked immunosorbent assay （EI.ISA） to detect anti-CCP antibody and the rate nephelometry to detect RF. Results In 187 cases of RA,AKA had the sensitivity of 58% and the specificity of 93%0 ,anti-CCP antibody had the sensitivity of 73% and the specificity of 95 %, and RF had the sensitivity of 67 % and the specificity of 77 %. Three joint detection had the sensitivity of 97 % and the specificity of 25 %. Conclusion The 3-item combination detection of AKA,anti-CCP and RF can improve the sensitivity in diagnosing RA.